Differential expression of poly(ADP-ribose) polymerase and DNA polymerase β in rat tissues

Marta Menegazzi, Gigliola Grassi-Zucconi, Alessandra Carcereri De Prati, Tsutomu Ogura, Palmiro Poltronieri, Hiroshi Nyunoya, Yuko Shiratori-Nyunoya, Masanao Miwa, Hisanori Suzuki

研究成果: Article査読

3 被引用数 (Scopus)


The activities of two DNA repair-related enzymes, poly(ADP-ribose) polymerase and DNA polymerase β, and their mRNA levels were measured in 17 tissues of Wistar rats. A large variety in enzyme activity values could be detected in the tissues examined; the highest levels of activity for both enzymes were found in the testis. A good correlation between poly(ADP-ribose) polymerase activity and the level of the transcript of the gene coding for the enzyme was observed in many tissues. A less satisfactory correlation could be evidenced for DNA polymerase β. The almost parallel amounts of the mRNAs for poly(ADP-ribose) polymerase and DNA polymerase β in the tissues examined suggest a possible coexpression of the genes coding for these enzymes. Additional studies have been carried out in testis and liver by immunohistochemical techniques and byin situ hybridization analyses. While in the testis the spermatocytes were shown to contain both enzymes and their transcripts, in other types of cells this could not be observed. In the liver mRNAs and enzymes were only found in 20% of the hepatocytes. This may in part explain both the low levels of the mRNAs and the modest activities of the two enzymes in that tissue.

ジャーナルExperimental Cell Research
出版ステータスPublished - 1991 11月

ASJC Scopus subject areas

  • 細胞生物学


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