TY - JOUR
T1 - Differential expression of poly(ADP-ribose) polymerase and DNA polymerase β in rat tissues
AU - Menegazzi, Marta
AU - Grassi-Zucconi, Gigliola
AU - De Prati, Alessandra Carcereri
AU - Ogura, Tsutomu
AU - Poltronieri, Palmiro
AU - Nyunoya, Hiroshi
AU - Shiratori-Nyunoya, Yuko
AU - Miwa, Masanao
AU - Suzuki, Hisanori
N1 - Funding Information:
The Authors thank Professor M. I,ibonatl and Dr. H. Esumi Ior their valuable advice in manuscript preparation, Dr. C. Cosi for brain preparations, and Mr. G. Righetti and Miss N. Takenouchi for skill full technical assistance. This work was partly supported by I he C.N.R. Target Project on Biotechnology and Bioinstrumentation. The authors are also deeply indebted to Dr. A. Mat.sukage for the kind gift of cDNA for rat DNA polymerase ~1 and of rabbit. anti-chicken DNA polymerase p antibody. Support by the (:entro per lo Studio delle Macromolecole Inf’ormazionali (CISMI, Milano. Pavia, Verona) is also gratefully acknowledged.
PY - 1991/11
Y1 - 1991/11
N2 - The activities of two DNA repair-related enzymes, poly(ADP-ribose) polymerase and DNA polymerase β, and their mRNA levels were measured in 17 tissues of Wistar rats. A large variety in enzyme activity values could be detected in the tissues examined; the highest levels of activity for both enzymes were found in the testis. A good correlation between poly(ADP-ribose) polymerase activity and the level of the transcript of the gene coding for the enzyme was observed in many tissues. A less satisfactory correlation could be evidenced for DNA polymerase β. The almost parallel amounts of the mRNAs for poly(ADP-ribose) polymerase and DNA polymerase β in the tissues examined suggest a possible coexpression of the genes coding for these enzymes. Additional studies have been carried out in testis and liver by immunohistochemical techniques and byin situ hybridization analyses. While in the testis the spermatocytes were shown to contain both enzymes and their transcripts, in other types of cells this could not be observed. In the liver mRNAs and enzymes were only found in 20% of the hepatocytes. This may in part explain both the low levels of the mRNAs and the modest activities of the two enzymes in that tissue.
AB - The activities of two DNA repair-related enzymes, poly(ADP-ribose) polymerase and DNA polymerase β, and their mRNA levels were measured in 17 tissues of Wistar rats. A large variety in enzyme activity values could be detected in the tissues examined; the highest levels of activity for both enzymes were found in the testis. A good correlation between poly(ADP-ribose) polymerase activity and the level of the transcript of the gene coding for the enzyme was observed in many tissues. A less satisfactory correlation could be evidenced for DNA polymerase β. The almost parallel amounts of the mRNAs for poly(ADP-ribose) polymerase and DNA polymerase β in the tissues examined suggest a possible coexpression of the genes coding for these enzymes. Additional studies have been carried out in testis and liver by immunohistochemical techniques and byin situ hybridization analyses. While in the testis the spermatocytes were shown to contain both enzymes and their transcripts, in other types of cells this could not be observed. In the liver mRNAs and enzymes were only found in 20% of the hepatocytes. This may in part explain both the low levels of the mRNAs and the modest activities of the two enzymes in that tissue.
UR - http://www.scopus.com/inward/record.url?scp=0026066660&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026066660&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(91)90480-I
DO - 10.1016/0014-4827(91)90480-I
M3 - Article
C2 - 1915664
AN - SCOPUS:0026066660
SN - 0014-4827
VL - 197
SP - 66
EP - 74
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -