TY - GEN
T1 - Direct immobilization of DNA on partially functionalized diamond surface
AU - Yang, Jung Hoon
AU - Song, Kwang Soup
AU - Kuga, Shouma
AU - Kawarada, Hiroshi
PY - 2006/1/1
Y1 - 2006/1/1
N2 - Amino groups were functionalized directly on the diamond surface after treating oxidation and fluorination for detection of DNAs, respectively. For simple process, immobilization of probe DNAs was carried out directly on the partially aminated diamond without linker molecules. After fabricating micropattemed diamond, specific hybridization with Cy-5 labeled target DNA at a concentration of 100 nM could be clearly detected on H-terminated, partially O-terminated, and partially F-terminated diamonds, respectively. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed.
AB - Amino groups were functionalized directly on the diamond surface after treating oxidation and fluorination for detection of DNAs, respectively. For simple process, immobilization of probe DNAs was carried out directly on the partially aminated diamond without linker molecules. After fabricating micropattemed diamond, specific hybridization with Cy-5 labeled target DNA at a concentration of 100 nM could be clearly detected on H-terminated, partially O-terminated, and partially F-terminated diamonds, respectively. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed.
UR - http://www.scopus.com/inward/record.url?scp=40949163548&partnerID=8YFLogxK
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U2 - 10.1557/proc-0950-d04-51
DO - 10.1557/proc-0950-d04-51
M3 - Conference contribution
AN - SCOPUS:40949163548
SN - 9781604234060
T3 - Materials Research Society Symposium Proceedings
SP - 220
EP - 227
BT - Materials Research Society Symposium Proceedings
PB - Materials Research Society
T2 - 2006 MRS Fall Meeting
Y2 - 27 November 2006 through 30 November 2006
ER -