Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

Hideki Itoh; Satoshi Arai; Thankiah Sudhaharan; Sung Chan Lee; Young Tae Chang; Shin'ichi Ishiwata; Madoka Suzuki; E. Birgitte Lane

doi:10.1039/c5cc09943a

Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

Hideki Itoh, Satoshi Arai, Thankiah Sudhaharan, Sung Chan Lee, Young Tae Chang, Shin'ichi Ishiwata^*, Madoka Suzuki, E. Birgitte Lane

^*この研究の対応する著者

理工学術院総合研究所

研究成果: Article › 査読

38 被引用数 (Scopus)

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We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca²⁺ burst.

本文言語	English
ページ（範囲）	4458-4461
ページ数	4
ジャーナル	Chemical Communications
巻	52
号	24
DOI	https://doi.org/10.1039/c5cc09943a
出版ステータス	Published - 2016

ASJC Scopus subject areas

化学 (全般)
触媒
セラミックおよび複合材料
電子材料、光学材料、および磁性材料
表面、皮膜および薄膜
材料化学
金属および合金

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abstract = "We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca2+ burst.",

author = "Hideki Itoh and Satoshi Arai and Thankiah Sudhaharan and Lee, {Sung Chan} and Chang, {Young Tae} and Shin'ichi Ishiwata and Madoka Suzuki and Lane, {E. Birgitte}",

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AU - Itoh, Hideki

AU - Arai, Satoshi

AU - Sudhaharan, Thankiah

AU - Lee, Sung Chan

AU - Chang, Young Tae

AU - Ishiwata, Shin'ichi

AU - Suzuki, Madoka

AU - Lane, E. Birgitte

PY - 2016

Y1 - 2016

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AB - We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca2+ burst.

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Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

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