Diurnal expression patterns of neurohypophysial hormone genes in the brain of the threespot wrasse Halichoeres trimaculatus

Sung Pyo Hur, Yuki Takeuchi, Yuriko Esaka, Wambiji Nina, Yong Ju Park, Hyeong Cheol Kang, Hyung Bok Jeong, Young Don Lee, Se Jae Kim, Akihiro Takemura*

*この研究の対応する著者

研究成果: Article査読

10 被引用数 (Scopus)

抄録

The aim of this study was to determine the involvement of neurohypophysial hormones in the diurnal patterns of the threespot wrasse Halichoeres trimaculatus, which is common in coral reefs and exhibits daily behavioral periodicity. Prohormone cDNAs of the neurohypophysial peptides, arginine vasotocin (AVT) and isotocin (IT), were cloned by 3′- and 5′-rapid amplification of cDNA ends (RACE). The distribution and expression patterns of pro-AVT and -IT mRNAs in the brain were determined using reverse-transcription polymerase chain reaction (RT-PCR) and real-time quantitative PCR, respectively. The respective full-length cDNAs of pro-AVT and -IT were 945 and 755. bp in length, respectively. The deduced amino acid sequences for pro-AVT and pro-IT were 154 and 156 residues in length, respectively. Both pro-peptides contained a signal sequence followed by the respective hormones and neurophysin connected by a Gly-Lys-Arg bridge. Pro-AVT mRNA was detected only in the hypothalamus area, while pro-IT mRNA in the whole part of the brain. The relative abundance of pro-AVT and -IT mRNA varied according to time of day; it was significantly greater at 12:00. h than at 24:00. h. Following intraperitoneal administration of melatonin, pro-AVT mRNA abundance in the brain decreased, while pro-IT mRNA abundance remained unchanged. These results demonstrate that daily fluctuations of pro-AVT and pro-IT levels in the brain of threespot wrasse are differentially regulated.

本文言語English
ページ(範囲)490-497
ページ数8
ジャーナルComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
158
4
DOI
出版ステータスPublished - 2011 4月
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 生理学

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