DNA conformation of the region preceding the beta-lactamase promoter of pUC19 which is required for efficient transcription.

Y. Hirota; T. Ohyama

DNA conformation of the region preceding the beta-lactamase promoter of pUC19 which is required for efficient transcription.

Y. Hirota^*, T. Ohyama

^*この研究の対応する著者

研究成果: Article › 査読

1 被引用数 (Scopus)

抄録

The upstream region of the beta-lactamase promoter of Escherichia coli plasmid pUC19 has a DNA curvature (bent DNA). This region was replaced with another sequence by using randomly synthesized oligonucleotides. Among the reconstructed plasmids, a plasmid which could endow E. coli cells with the strongest resistance to ampicillin on plates was selected. Nucleotide sequence and DNA conformation of the altered region was investigated.

本文言語	English
ページ（範囲）	151-152
ページ数	2
ジャーナル	Nucleic acids symposium series
号	27
出版ステータス	Published - 1992
外部発表	はい

ASJC Scopus subject areas

医学（全般）

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引用スタイル

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abstract = "The upstream region of the beta-lactamase promoter of Escherichia coli plasmid pUC19 has a DNA curvature (bent DNA). This region was replaced with another sequence by using randomly synthesized oligonucleotides. Among the reconstructed plasmids, a plasmid which could endow E. coli cells with the strongest resistance to ampicillin on plates was selected. Nucleotide sequence and DNA conformation of the altered region was investigated.",

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AU - Hirota, Y.

AU - Ohyama, T.

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N2 - The upstream region of the beta-lactamase promoter of Escherichia coli plasmid pUC19 has a DNA curvature (bent DNA). This region was replaced with another sequence by using randomly synthesized oligonucleotides. Among the reconstructed plasmids, a plasmid which could endow E. coli cells with the strongest resistance to ampicillin on plates was selected. Nucleotide sequence and DNA conformation of the altered region was investigated.

AB - The upstream region of the beta-lactamase promoter of Escherichia coli plasmid pUC19 has a DNA curvature (bent DNA). This region was replaced with another sequence by using randomly synthesized oligonucleotides. Among the reconstructed plasmids, a plasmid which could endow E. coli cells with the strongest resistance to ampicillin on plates was selected. Nucleotide sequence and DNA conformation of the altered region was investigated.

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JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

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