TY - JOUR
T1 - Effect of inorganic carbon limitation on the nitrogen removal performance of the single-stage reactor containing anammox and nitritation gel carriers
AU - Isaka, Kazuichi
AU - Nitta, Shiori
AU - Osaka, Toshifumi
AU - Tsuneda, Satoshi
N1 - Publisher Copyright:
© 2021 The Society for Biotechnology, Japan
PY - 2022/1
Y1 - 2022/1
N2 - Herein, the effect of inorganic carbon (IC) limitation on the nitrogen removal performance of the single-stage reactor containing nitritation and anammox gel carriers was investigated. As a result of a continuous feeding test, the effluent ammonium concentration increased as the IC concentration decreased, indicating the deterioration of nitritation activity, not anammox. Furthermore, the sensitivity of IC to anammox and nitritation activity was investigated in anammox and nitritation reactors, respectively. Consequently, the relationship between the effluent IC concentration and nitritation rate was well described using the Michaelis–Menten equation. The apparent Km value of nitritation was calculated as 4.4 mg-C L−1. In anammox reactor, it was calculated as 1.7 mg-C L−1. These results revealed that the affinity of nitritation gel carriers to IC was lower than that of anammox, supporting that nitritation activity was easily deactivated by decrease in the IC concentration rather than anammox. Microbial community analysis revealed that Nitrosomonas europaea and Candidatus Jettenia asiatica were the dominant species of ammonium-oxidizing and anammox bacteria.
AB - Herein, the effect of inorganic carbon (IC) limitation on the nitrogen removal performance of the single-stage reactor containing nitritation and anammox gel carriers was investigated. As a result of a continuous feeding test, the effluent ammonium concentration increased as the IC concentration decreased, indicating the deterioration of nitritation activity, not anammox. Furthermore, the sensitivity of IC to anammox and nitritation activity was investigated in anammox and nitritation reactors, respectively. Consequently, the relationship between the effluent IC concentration and nitritation rate was well described using the Michaelis–Menten equation. The apparent Km value of nitritation was calculated as 4.4 mg-C L−1. In anammox reactor, it was calculated as 1.7 mg-C L−1. These results revealed that the affinity of nitritation gel carriers to IC was lower than that of anammox, supporting that nitritation activity was easily deactivated by decrease in the IC concentration rather than anammox. Microbial community analysis revealed that Nitrosomonas europaea and Candidatus Jettenia asiatica were the dominant species of ammonium-oxidizing and anammox bacteria.
KW - Affinity
KW - Anammox
KW - Gel
KW - Inorganic carbon
KW - Nitrification
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U2 - 10.1016/j.jbiosc.2021.09.014
DO - 10.1016/j.jbiosc.2021.09.014
M3 - Article
C2 - 34688558
AN - SCOPUS:85119112110
SN - 1389-1723
VL - 133
SP - 70
EP - 75
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 1
ER -