Effect of mechanical stretch on TGF-β1 expression of C2C12 myogenic cells

Takayuki Akimoto; Takashi Ushida; Shigeru Miyaki; Hiroshi Akaogi; Tetsuya Tateishi; Toru Fukubayashi

doi:10.1016/j.msec.2003.12.013

Effect of mechanical stretch on TGF-β1 expression of C2C12 myogenic cells

Takayuki Akimoto^*, Takashi Ushida, Shigeru Miyaki, Hiroshi Akaogi, Tetsuya Tateishi, Toru Fukubayashi

^*この研究の対応する著者

研究成果: Article › 査読

3 被引用数 (Scopus)

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The purpose of this study was to examine whether mechanical stretch altered the expression of transforming growth factor (TGF)-β1 in cultured myogenic satellite cell. C2C12 myogenic satellite cell was plated on collagenized silastic membrane and cultured in growth medium and stretched 20% elongation for 24 h. The expression of TGF-β1 was determined by semiquantitive reverse transcriptional polymerase chain reaction (RT-PCR) analysis. Mechanical stretch decreased myogenic regulatory factors. The expression of TGF-β1 was increased in stretched C2C12 as compared to control. This result demonstrates that mechanical stretch inhibits myogenic differentiation by an increase of TGF-β1 expression on C2C12 myogenic satellite cell.

本文言語	English
ページ（範囲）	387-389
ページ数	3
ジャーナル	Materials Science and Engineering C
巻	24
号	3
DOI	https://doi.org/10.1016/j.msec.2003.12.013
出版ステータス	Published - 2004 4月 1
外部発表	はい

ASJC Scopus subject areas

材料科学（全般）
凝縮系物理学
材料力学
機械工学

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10.1016/j.msec.2003.12.013

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title = "Effect of mechanical stretch on TGF-β1 expression of C2C12 myogenic cells",

abstract = "The purpose of this study was to examine whether mechanical stretch altered the expression of transforming growth factor (TGF)-β1 in cultured myogenic satellite cell. C2C12 myogenic satellite cell was plated on collagenized silastic membrane and cultured in growth medium and stretched 20% elongation for 24 h. The expression of TGF-β1 was determined by semiquantitive reverse transcriptional polymerase chain reaction (RT-PCR) analysis. Mechanical stretch decreased myogenic regulatory factors. The expression of TGF-β1 was increased in stretched C2C12 as compared to control. This result demonstrates that mechanical stretch inhibits myogenic differentiation by an increase of TGF-β1 expression on C2C12 myogenic satellite cell.",

keywords = "C2C12 myogenic cells, Mechanical stretch, TGF-β1 expression",

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T1 - Effect of mechanical stretch on TGF-β1 expression of C2C12 myogenic cells

AU - Akimoto, Takayuki

AU - Ushida, Takashi

AU - Miyaki, Shigeru

AU - Akaogi, Hiroshi

AU - Tateishi, Tetsuya

AU - Fukubayashi, Toru

PY - 2004/4/1

Y1 - 2004/4/1

N2 - The purpose of this study was to examine whether mechanical stretch altered the expression of transforming growth factor (TGF)-β1 in cultured myogenic satellite cell. C2C12 myogenic satellite cell was plated on collagenized silastic membrane and cultured in growth medium and stretched 20% elongation for 24 h. The expression of TGF-β1 was determined by semiquantitive reverse transcriptional polymerase chain reaction (RT-PCR) analysis. Mechanical stretch decreased myogenic regulatory factors. The expression of TGF-β1 was increased in stretched C2C12 as compared to control. This result demonstrates that mechanical stretch inhibits myogenic differentiation by an increase of TGF-β1 expression on C2C12 myogenic satellite cell.

AB - The purpose of this study was to examine whether mechanical stretch altered the expression of transforming growth factor (TGF)-β1 in cultured myogenic satellite cell. C2C12 myogenic satellite cell was plated on collagenized silastic membrane and cultured in growth medium and stretched 20% elongation for 24 h. The expression of TGF-β1 was determined by semiquantitive reverse transcriptional polymerase chain reaction (RT-PCR) analysis. Mechanical stretch decreased myogenic regulatory factors. The expression of TGF-β1 was increased in stretched C2C12 as compared to control. This result demonstrates that mechanical stretch inhibits myogenic differentiation by an increase of TGF-β1 expression on C2C12 myogenic satellite cell.

KW - C2C12 myogenic cells

KW - Mechanical stretch

KW - TGF-β1 expression

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DO - 10.1016/j.msec.2003.12.013

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JO - Materials Science and Engineering C

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Effect of mechanical stretch on TGF-β1 expression of C2C12 myogenic cells

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