TY - JOUR
T1 - Effect of vitamin C depletion on age-related hearing loss in SMP30/GNL knockout mice
AU - Kashio, Akinori
AU - Amano, Akiko
AU - Kondo, Yoshitake
AU - Sakamoto, Takashi
AU - Iwamura, Hitoshi
AU - Suzuki, Mitsuya
AU - Ishigami, Akihito
AU - Yamasoba, Tatsuya
PY - 2009/12/18
Y1 - 2009/12/18
N2 - Using senescence marker protein 30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), we examined whether modulating VC level affects age-related hearing loss (AHL). KO and wild-type (WT) C57BL/6 mice were given water containing 1.5 g/L VC [VC(+)] or 37.5 mg/L VC [VC(-)]. At 10 months of age, KO VC(-) mice showed significant reduction in VC level in the inner ear, plasma, and liver, increase in auditory brainstem response (ABR) thresholds, and decrease in the number of spiral ganglion cells compared to WT VC(-), WT VC(+), and KO VC(+) mice. There were no differences in VC level in the inner ear, ABR thresholds, or the number of spiral ganglion cells among WT VC(-), WT VC(+), and KO VC(+) mice. These findings suggest that VC depletion can accelerate AHL but that supplementing VC may not increase VC level in the inner ear or slow AHL in mice.
AB - Using senescence marker protein 30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), we examined whether modulating VC level affects age-related hearing loss (AHL). KO and wild-type (WT) C57BL/6 mice were given water containing 1.5 g/L VC [VC(+)] or 37.5 mg/L VC [VC(-)]. At 10 months of age, KO VC(-) mice showed significant reduction in VC level in the inner ear, plasma, and liver, increase in auditory brainstem response (ABR) thresholds, and decrease in the number of spiral ganglion cells compared to WT VC(-), WT VC(+), and KO VC(+) mice. There were no differences in VC level in the inner ear, ABR thresholds, or the number of spiral ganglion cells among WT VC(-), WT VC(+), and KO VC(+) mice. These findings suggest that VC depletion can accelerate AHL but that supplementing VC may not increase VC level in the inner ear or slow AHL in mice.
KW - Aging
KW - Ascorbic acid
KW - Cochlea
KW - Gluconolactonase
KW - Hearing loss
KW - Oxidative stress
KW - Senescence marker protein 30
KW - Vitamin C
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UR - http://www.scopus.com/inward/citedby.url?scp=70449708941&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2009.09.003
DO - 10.1016/j.bbrc.2009.09.003
M3 - Article
C2 - 19733551
AN - SCOPUS:70449708941
SN - 0006-291X
VL - 390
SP - 394
EP - 398
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -