Effects of 2-deoxy-d-glucose and quinidine on the fruiting body formation in Coprinus Macrorhizus

The addition of 2-deoxy-D-glucose and quinidine to culture media of strain Fis^c of Coprinus macrorhizus was effective in inhibiting fruiting body formation and in reducing the intracellular level of cyclic AMP, adenylate cyclase and phosphodiesterase activities. 2-Deoxy-D-glucose taken into mycelial cells was phosphorylated and accumulated as 2-deoxy-D-glucose-monophosphate and 2-deoxy-D-glucose diphosphate. This phosphorylation appeared to be reversible. Quinidine was taken up by mycelial cells without further metabolism. 2-Deoxy-D-glucose, 2-deoxy-D-glucosemono-phosphate and quinidine inhibited adenylate cyclase activity, but not phosphodiesterase activity measured in vitro. The K_i values of 2-deoxy-D-glucose and quinidine for adenylate cyclase activity were 1.8 and 0.08 mM, respectively. The data indicate a possible relationship between the low adenylate cyclase activity and inhibition of fruiting body formation.