Effects of carbocisteine on sialyl-Lewis x expression in an airway carcinoma cell line stimulated with tumor necrosis factor-α

Yuji Ishibashi*, Shigeru Imai, Yoshio Inouye, Teruo Okano, Akiyoshi Taniguchi

*この研究の対応する著者

研究成果: Article査読

16 被引用数 (Scopus)

抄録

Carbocisteine is a mucoregulatory drug normalizing sialic acid and fucose contents in mucins through the regulation of glycosyltransferase activities. Tumor necrosis factor (TNF)-α-induced overexpression of sialyl-Lewis x epitopes, containing sialic acid and fucose, in mucins were previously reported to be regulated by glycosyltransferase mRNAs expression through phosphatidyl inositol-specific phospholipase C (PI-PLC) signaling pathways [Ishibashi, Y., Inouye, Y., Okano, T., Taniguchi, A., 2005. Regulation of sialyl-Lewis x epitope expression by TNF-α and EGF in an airway carcinoma cell line. Glycoconj. J. 22, 53-62]. To investigate the mechanism behind the mucoregulatory action of carbocisteine, the present study evaluated the effects of carbocisteine on TNF-α-induced overexpression of sialyl-Lewis x epitopes in NCI-H292 cells. 100 μg/ml of carbocisteine was able to inhibit the TNF-α-induced expression of hST3GallV mRNA, FUT3 mRNA, C2/4GnT mRNA and sialyl-Lewis x epitopes as well as the TNF-α-induced activity of PI-PLC in NCI-H292 cells. These findings suggest that carbocisteine may normalize the sialyl-Lewis x epitopes expression in mucins through the inhibition of cellular PI-PLC activity in vivo.

本文言語English
ページ(範囲)223-228
ページ数6
ジャーナルEuropean Journal of Pharmacology
530
3
DOI
出版ステータスPublished - 2006 1月 20
外部発表はい

ASJC Scopus subject areas

  • 薬理学

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