TY - JOUR
T1 - Effects of carriers on transgene expression from plasmids containing a DNA sequence with high histone affinity
AU - Kamiya, Hiroyuki
AU - Fukunaga, Satoki
AU - Ohyama, Takashi
AU - Harashima, Hideyoshi
N1 - Funding Information:
This work was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan, from the Japan Society for the Promotion of Science, and from the Akiyama Foundation.
PY - 2009/7/6
Y1 - 2009/7/6
N2 - The intranuclear disposition of plasmid DNA is highly important for transgene expression. The effects of a left-handedly curved sequence with high histone affinity on transgene expression were examined in COS-7 cells with two kinds of carriers (Lipofectamine Plus and TransIT-LT1). Three plasmids containing the curved sequence at different positions were transfected. The transgene expression was affected by the position of the left-handedly curved sequence, and the sequence at appropriate locations enhanced the expression from plasmid DNAs. However, the position effects on the expression differed from those obtained by electroporation of the same plasmid DNAs in a naked form. In addition, the degree of expression enhancement seemed to depend on the carriers. These results suggest that the left-handedly curved sequence with high histone affinity could increase the transgene expression from a plasmid delivered with carriers.
AB - The intranuclear disposition of plasmid DNA is highly important for transgene expression. The effects of a left-handedly curved sequence with high histone affinity on transgene expression were examined in COS-7 cells with two kinds of carriers (Lipofectamine Plus and TransIT-LT1). Three plasmids containing the curved sequence at different positions were transfected. The transgene expression was affected by the position of the left-handedly curved sequence, and the sequence at appropriate locations enhanced the expression from plasmid DNAs. However, the position effects on the expression differed from those obtained by electroporation of the same plasmid DNAs in a naked form. In addition, the degree of expression enhancement seemed to depend on the carriers. These results suggest that the left-handedly curved sequence with high histone affinity could increase the transgene expression from a plasmid delivered with carriers.
KW - Carriers
KW - Histones
KW - Intranuclear disposition
KW - Left-handedly curved DNA
KW - Plasmid DNA
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U2 - 10.1016/j.ijpharm.2009.04.032
DO - 10.1016/j.ijpharm.2009.04.032
M3 - Article
C2 - 19409470
AN - SCOPUS:67349193206
SN - 0378-5173
VL - 376
SP - 99
EP - 103
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
IS - 1-2
ER -