@inbook{09656bbb28c748ec9fa5c6e3203949fd,
title = "Efficient depletion of fission yeast condensin by combined transcriptional repression and auxin-induced degradation",
abstract = "Structural maintenance of chromosomes (SMC) complexes play pivotal roles in controlling chromatin organization. Condensin is an essential SMC complex that compacts chromatin to form condensed chromosomes in mitosis. Complete condensin inactivation is necessary to reveal how condensin converts interphase chromatin into mitotic chromosomes. Here, we have developed a condensin depletion system in fission yeast that combines transcriptional repression with auxin-inducible protein degradation. This achieves efficient condensin depletion without need for a temperature shift. Our system is useful when studying how condensin contributes to chromosome architecture and is applicable to the study of other SMC complexes.",
keywords = "Auxin-inducible degron, Chromosome condensation, Condensin, Fission yeast, SMC complex, Transcriptional repression",
author = "Yasutaka Kakui and Frank Uhlmann",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9520-2_3",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "25--33",
booktitle = "Methods in Molecular Biology",
}