TY - GEN
T1 - EFFICIENT PROTEIN CRYSTALLIZAITON AND DAMAGELESS EXTRACTION FLOW DEVICE USING MULTI-MICRODROPLET TRAPPING STRUCTURE
AU - Miyazaki, A.
AU - Tanaka, D.
AU - Sekiguchi, Tetsushi
AU - Furuya, M.
AU - Shoji, S.
N1 - Publisher Copyright:
© 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2022
Y1 - 2022
N2 - We proposed an efficient protein crystallization device that applied a multi-microdroplet trapping method[1]. 30 protein contained microdroplets were trapped and multiple protein crystallization were performed at the same time. The device was fabricated by a flow channel formed PDMS and a PDMS coated glass substrate. In order to obtain damageless extraction of generated protein crystal, PDMS-PDMS bonding strength was controlled by optimizing composition of PDMS and curing condition. Protein crystallization was observed in 60 percent of trapped microdroplets within six hours. 100 μm lysozyme crystals were extracted from the device without damage.
AB - We proposed an efficient protein crystallization device that applied a multi-microdroplet trapping method[1]. 30 protein contained microdroplets were trapped and multiple protein crystallization were performed at the same time. The device was fabricated by a flow channel formed PDMS and a PDMS coated glass substrate. In order to obtain damageless extraction of generated protein crystal, PDMS-PDMS bonding strength was controlled by optimizing composition of PDMS and curing condition. Protein crystallization was observed in 60 percent of trapped microdroplets within six hours. 100 μm lysozyme crystals were extracted from the device without damage.
KW - Easy extraction of products
KW - Efficient protein crystallization
KW - Multi microdroplet trapping device
UR - http://www.scopus.com/inward/record.url?scp=85175695451&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85175695451&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85175695451
T3 - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 765
EP - 766
BT - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
Y2 - 23 October 2022 through 27 October 2022
ER -