Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer

Tetsushi Taguchi; Liming Xu; Hisatoshi Kobayashi; Akiyoshi Taniguchi; Kazunori Kataoka; Junzo Tanaka

doi:10.1016/j.biomaterials.2004.04.029

Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer

Tetsushi Taguchi^*, Liming Xu, Hisatoshi Kobayashi, Akiyoshi Taniguchi, Kazunori Kataoka, Junzo Tanaka

^*この研究の対応する著者

研究成果: Article › 査読

69 被引用数 (Scopus)

抄録

An in situ gel system was developed to encapsulate chondrocytes under physiological conditions using an alkali-treated collagen (AlCol) and pentaerythritol poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-PEG) as a crosslinker. AlCol gels were obtained at crosslinker concentrations from 0.1 to 3.0mM. Chondrocytes were encapsulated and dispersed homogeneously in AlCol gels. Results of MTT staining showed that cells survived after encapsulation in AlCol gels. Biochemical analysis demonstrated that DNA content in AlCol gels was constant after 3 weeks. Glycosaminoglycan content and mRNA expression of type II collagen and aggrecan increased with culture time. These results suggest that this in situ gel system is useful for regenerating cartilage in vitro and for minimally invasive therapy for cartilage defects.

本文言語	English
ページ（範囲）	1247-1252
ページ数	6
ジャーナル	Biomaterials
巻	26
号	11
DOI	https://doi.org/10.1016/j.biomaterials.2004.04.029
出版ステータス	Published - 2005 4月
外部発表	はい

ASJC Scopus subject areas

バイオエンジニアリング
セラミックおよび複合材料
生物理学
生体材料
材料力学

文献へのアクセス

10.1016/j.biomaterials.2004.04.029

他のファイルとリンク

フィンガープリント

「Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

@article{fab770994fc9459bab64a086a68e4952,

title = "Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer",

abstract = "An in situ gel system was developed to encapsulate chondrocytes under physiological conditions using an alkali-treated collagen (AlCol) and pentaerythritol poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-PEG) as a crosslinker. AlCol gels were obtained at crosslinker concentrations from 0.1 to 3.0mM. Chondrocytes were encapsulated and dispersed homogeneously in AlCol gels. Results of MTT staining showed that cells survived after encapsulation in AlCol gels. Biochemical analysis demonstrated that DNA content in AlCol gels was constant after 3 weeks. Glycosaminoglycan content and mRNA expression of type II collagen and aggrecan increased with culture time. These results suggest that this in situ gel system is useful for regenerating cartilage in vitro and for minimally invasive therapy for cartilage defects.",

keywords = "Alkali-treated collagen, Chondrocytes, Polyethylene glycol, Star polymer",

author = "Tetsushi Taguchi and Liming Xu and Hisatoshi Kobayashi and Akiyoshi Taniguchi and Kazunori Kataoka and Junzo Tanaka",

note = "Funding Information: We thank Drs. Y. Mandai and Y. Hiraoka of Nitta Gelatin Co. Ltd., for their invaluable advice and Ms. K. Tateno of Biomaterials Center, National Institute for Materials Science, for technical support. This work was financially supported in part by Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST).",

year = "2005",

month = apr,

doi = "10.1016/j.biomaterials.2004.04.029",

language = "English",

volume = "26",

pages = "1247--1252",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier BV",

number = "11",

}

TY - JOUR

T1 - Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer

AU - Taguchi, Tetsushi

AU - Xu, Liming

AU - Kobayashi, Hisatoshi

AU - Taniguchi, Akiyoshi

AU - Kataoka, Kazunori

AU - Tanaka, Junzo

N1 - Funding Information: We thank Drs. Y. Mandai and Y. Hiraoka of Nitta Gelatin Co. Ltd., for their invaluable advice and Ms. K. Tateno of Biomaterials Center, National Institute for Materials Science, for technical support. This work was financially supported in part by Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST).

PY - 2005/4

Y1 - 2005/4

N2 - An in situ gel system was developed to encapsulate chondrocytes under physiological conditions using an alkali-treated collagen (AlCol) and pentaerythritol poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-PEG) as a crosslinker. AlCol gels were obtained at crosslinker concentrations from 0.1 to 3.0mM. Chondrocytes were encapsulated and dispersed homogeneously in AlCol gels. Results of MTT staining showed that cells survived after encapsulation in AlCol gels. Biochemical analysis demonstrated that DNA content in AlCol gels was constant after 3 weeks. Glycosaminoglycan content and mRNA expression of type II collagen and aggrecan increased with culture time. These results suggest that this in situ gel system is useful for regenerating cartilage in vitro and for minimally invasive therapy for cartilage defects.

AB - An in situ gel system was developed to encapsulate chondrocytes under physiological conditions using an alkali-treated collagen (AlCol) and pentaerythritol poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-PEG) as a crosslinker. AlCol gels were obtained at crosslinker concentrations from 0.1 to 3.0mM. Chondrocytes were encapsulated and dispersed homogeneously in AlCol gels. Results of MTT staining showed that cells survived after encapsulation in AlCol gels. Biochemical analysis demonstrated that DNA content in AlCol gels was constant after 3 weeks. Glycosaminoglycan content and mRNA expression of type II collagen and aggrecan increased with culture time. These results suggest that this in situ gel system is useful for regenerating cartilage in vitro and for minimally invasive therapy for cartilage defects.

KW - Alkali-treated collagen

KW - Chondrocytes

KW - Polyethylene glycol

KW - Star polymer

UR - http://www.scopus.com/inward/record.url?scp=4744353723&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4744353723&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2004.04.029

DO - 10.1016/j.biomaterials.2004.04.029

M3 - Article

C2 - 15475054

AN - SCOPUS:4744353723

SN - 0142-9612

VL - 26

SP - 1247

EP - 1252

JO - Biomaterials

JF - Biomaterials

IS - 11

ER -