TY - GEN
T1 - Endothelial cell marker gene expression on double-layered co-culture utilizing thermo-responsive culture dish
AU - Shirai, Y.
AU - Kurosawa, Y.
AU - Okano, T.
AU - Taniguchi, A.
PY - 2004/12/1
Y1 - 2004/12/1
N2 - Endothelial cell marker gene expression on the reconstruction of double-layered co-culture endothelial cells was investigated using thermo-responsive culture dish. Human umbilical vein endothelial cells (HUVEC) at passage 3 were cultured in modified EGM-2 for the experiments. The gene expression levels of PECAM-1, ICAM-1 and Factor VIII on double-layered co-culture endothelial cells were increased after 2 weeks compared with monolayer culture. The results suggests that hepatocyte functions and endothelial cell functions are maintained and liver parenchyma during double-layered co-culture.
AB - Endothelial cell marker gene expression on the reconstruction of double-layered co-culture endothelial cells was investigated using thermo-responsive culture dish. Human umbilical vein endothelial cells (HUVEC) at passage 3 were cultured in modified EGM-2 for the experiments. The gene expression levels of PECAM-1, ICAM-1 and Factor VIII on double-layered co-culture endothelial cells were increased after 2 weeks compared with monolayer culture. The results suggests that hepatocyte functions and endothelial cell functions are maintained and liver parenchyma during double-layered co-culture.
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M3 - Conference contribution
AN - SCOPUS:13844276405
SN - 1877040193
SN - 9781877040191
T3 - Transactions - 7th World Biomaterials Congress
BT - Transactions - 7th World Biomaterials Congress
T2 - Transactions - 7th World Biomaterials Congress
Y2 - 17 May 2004 through 21 May 2004
ER -