抄録
An efficient method for replacement of nucleotide sequences in the D-loop of T. utilis tRNATyr has been developed. An abnormal tRNATyr lacking in tetranucleotide D16-D-Gm-G19 in its D-loop has been reconstructed by this method and shown to accept tyrosine to about 55% of the aminoacylation level observed for intact tRNATyr. This suggests that the deleted sequence itself is not essential for recognition by TyrRS but a conformational instability of the tRNA possibly caused by the disruption of tertiary interactions between the D-loop and T psi C-loop might have influenced the forward reaction rate leading to the decreased level of aminoacylation.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 137-140 |
| ページ数 | 4 |
| ジャーナル | Nucleic acids symposium series |
| 号 | 12 |
| 出版ステータス | Published - 1983 12月 1 |
| 外部発表 | はい |
ASJC Scopus subject areas
- 医学（全般）