Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts

Sayaka Wakayama, Martin L. Jakt, Masako Suzuki, Ryoko Araki, Takafusa Hikichi, Satoshi Kishigami, Hiroshi Ohta, Nguyen Van Thuan, Eiji Mizutani, Yuko Sakaide, Sho Senda, Satoshi Tanaka, Mitsuhiro Okada, Masashi Miyake, Masumi Abe, Shin Ichi Nishikawa, Kunio Shiota, Teruhiko Wakayama*

*この研究の対応する著者

研究成果: Article査読

149 被引用数 (Scopus)

抄録

Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT-ESCs have abnormalities, such as those associated with the offspring produced by reproductive cloning, their scientific and medical utilities might prove limited. To evaluate the characteristics of NT-ESCs, we established more than 150 NT-ESC lines from adult somatic cells of several mouse strains. Here, we show that these NT-ESCs were able to differentiate into all functional embryonic tissues in vivo. Moreover, they were identical to blastocyst-derived ESCs in terms of their expression of pluripotency markers in the presence of tissue-dependent differentially DNA methylated regions, in DNA microarray profiles, and in high-coverage gene expression profiling. Importantly, the NT procedure did not cause irreversible damage to the nuclei. These similarities of NT-ESCs and ESCs indicate that murine therapeutic cloning by somatic cell NT can provide a reliable model for preclinical stem cell research.

本文言語English
ページ(範囲)2023-2033
ページ数11
ジャーナルStem Cells
24
9
DOI
出版ステータスPublished - 2006
外部発表はい

ASJC Scopus subject areas

  • 細胞生物学

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