@article{faa37b1f8ebe4540afad5adaa7c24281,
title = "Establishment and characterization of highly osteolytic luminal breast cancer cell lines by intracaudal arterial injection",
abstract = "Bone is one of the most common metastatic sites of breast cancer, and bone metastasis profoundly affects the quality of life of breast cancer patients. Bone metastasis is commonly observed among all the subtypes of breast cancer; however, its molecular mechanism has been analyzed only in triple-negative subtype of breast cancer (TNBC). To characterize the molecular mechanisms of bone metastasis of luminal breast cancer, we established a bone-metastatic model of the MCF7, luminal breast cancer cell line, with enhanced osteolytic activity by intracaudal arterial injection (CAI). Pathological analysis of the established cell lines revealed that they exhibited fierce osteolytic ability by promoting osteoclast differentiation and activity. The signature genes extracted from highly osteolytic MCF7 cell lines were differed from those of bone-metastatic TNBC cell lines. Our results suggest that unique mechanisms of osteolysis in bone-metastatic lesions of luminal breast cancer. In addition, several up-regulated genes in MCF7-BM (Bone Metastasis) 02 cell lines correlated with poor prognosis with luminal breast cancer patients. Our findings support further study on the bone-metastatic mechanisms of luminal breast cancer.",
keywords = "MCF7, bone metastasis, intracaudal arterial injection, luminal breast cancer, microarray, osteolysis",
author = "Yuxuan Han and Jun Nakayama and Yusuke Hayashi and Seongmoon Jeong and Mitsuru Futakuchi and Emi Ito and Shinya Watanabe and Kentaro Semba",
note = "Funding Information: We thank Prof. A. Kudo from the Tokyo Institute of Technology for provision of M-CSF. We also thank Prof. J. Inoue and Dr. Y. Taguchi for providing mRANKL and the TRAcP assay protocol. Histological analyses in this work were supported by Pathologic Analysis in JSPS KAKENHI (Grant No. 16H06276), Grant-in-Aid for Scientific Research on Innovative Areas—Platforms for Advanced Technologies and Research Resources. We thank H. Nikki March, PhD, from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript. Funding Information: We thank Prof. A. Kudo from the Tokyo Institute of Technology for provision of M‐CSF. We also thank Prof. J. Inoue and Dr. Y. Taguchi for providing mRANKL and the TRAcP assay protocol. Histological analyses in this work were supported by Pathologic Analysis in JSPS KAKENHI (Grant No. 16H06276), Grant‐in‐Aid for Scientific Research on Innovative Areas—Platforms for Advanced Technologies and Research Resources. We thank H. Nikki March, PhD, from Edanz Group ( www.edanzediting.com/ac ) for editing a draft of this manuscript. Publisher Copyright: {\textcopyright} 2019 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd",
year = "2020",
month = feb,
day = "1",
doi = "10.1111/gtc.12743",
language = "English",
volume = "25",
pages = "111--123",
journal = "Genes to Cells",
issn = "1356-9597",
publisher = "Wiley-Blackwell",
number = "2",
}