Expression of activities of two 20α-hydroxysteroid dehydrogenase isozymes in rat corpora lutea

H. H. Seong*, K. Shiota, K. Noda, A. Ogura, T. Asano, M. Takahashi


研究成果: Article査読

18 被引用数 (Scopus)


The rat ovary contains two isozymes of 20α-hydroxysteroid dehydrogenase (HSD-1 and HSD-2). In this study, the expression of activity of each isozyme was investigated in ovaries that contained a single generation of corpora lutea during pseudopregnancy. This condition was induced by cervical stimulation in rats that had been rendered anovulatory by housing them in a continuously lit environment. The total activity of cytosolic 20α-HSD was lower in the ovaries of these pseudopregnant rats than in ovaries containing multiple generations of corpora lutea. In normal pseudopregnancy, HSD-1 activity was low on days 5 and 9 and increased markedly on day 15, whereas HSD-2 was lower than HSD-1 and did not vary throughout pseudopregnancy. However, on days 5 and 9 of continuous-light pseudopregnancy, low activity of HSD-1 only was detected; by day 15, HSD-1 activity had increased sixfold and HSD-2 activity could be detected. Immunohistochemical methods using a specific antibody recognizing both HSD-1 and HSD-2 revealed that the number of 20α-HSD-positive luteal cells increased by day 15. Thus, the increase in total enzyme activity and appearance of HSD-2 activity observed at late pseudopregnancy was accompanied by an increase in the number of 20α-HSD-positive luteal cells.

ジャーナルJournal of Reproduction and Fertility
出版ステータスPublished - 1992

ASJC Scopus subject areas

  • 発生生物学
  • 分子生物学
  • 生理学
  • 胎生学
  • 産婦人科学


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