@article{611356c3e2b84fcda40d963febd2f234,
title = "Expression screening of 17q12-21 amplicon reveals GRB7 as an ERBB2-dependent oncogene",
abstract = "Gene amplification is a major genetic alteration in human cancers. Amplicons, amplified genomic regions, are believed to contain {"}driver{"} genes responsible for tumorigenesis. However, the significance of co-amplified genes has not been extensively studied. We have established an integrated analysis system of amplicons using retrovirus-mediated gene transfer coupled with a human full-length cDNA set. Applying this system to 17q12-21 amplicon observed in breast cancer, we identified GRB7 as a context-dependent oncogene, which modulates the ERBB2 signaling pathway through enhanced phosphorylation of ERBB2 and Akt. Our work provides an insight into the biological significance of gene amplification in human cancers.",
keywords = "ERBB2, Expression screening, GRB7, Gene amplification, Tumorigenesis",
author = "Makoto Saito and Yukiko Kato and Emi Ito and Jiro Fujimoto and Kosuke Ishikawa and Ayano Doi and Kentaro Kumazawa and Atsuka Matsui and Shiori Takebe and Takaomi Ishida and Sakura Azuma and Hiromi Mochizuki and Yoshifumi Kawamura and Yuka Yanagisawa and Reiko Honma and Imai, {Jun Ichi} and Hirokazu Ohbayashi and Naoki Goshima and Kentaro Semba and Shinya Watanabe",
note = "Funding Information: We thank Kumiko Semba for her secretarial assistance. This research was partially supported by JSPS KAKENHI 23241064 and a grant for translational research programs from New Energy and Industrial Technology Development Organization (NEDO). ",
year = "2012",
month = jun,
day = "12",
doi = "10.1016/j.febslet.2012.05.003",
language = "English",
volume = "586",
pages = "1708--1714",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "12",
}