Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system

Muneki Isokawa; Yanting Song; Katsuya Takatsuki; Tetsushi Sekiguchi; Jun Mizuno; Takashi Funatsu; Shuichi Shoji; Makoto Tsunoda

Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system

Muneki Isokawa, Yanting Song, Katsuya Takatsuki, Tetsushi Sekiguchi, Jun Mizuno, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda^*

^*この研究の対応する著者

ナノ・ライフ創新研究機構

研究成果: Conference contribution

抄録

A rapid and quantitative method for the determination of phenylalanine (Phe) and tyrosine (Tyr) in plasma using pressure-driven liquid chromatography (LC) on a chip was developed. After derivatizing Phe and Tyr with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), separation was performed within 140 s on a previously developed integration chip with a pillar array column and a gradient elution system [1,2]. The Phe and Tyr in plasma were quantified and good validation data were obtained. As Phe and Tyr are considered to be important biomarkers of some diseases, especially for newborn screening, the developed method is promising for rapid diagnosis in the clinical field.

本文言語	English
ホスト出版物のタイトル	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
出版社	Chemical and Biological Microsystems Society
ページ	941-943
ページ数	3
ISBN（印刷版）	9780979806476
出版ステータス	Published - 2014
イベント	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States 継続期間: 2014 10月 26 → 2014 10月 30

Other

Other	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
国/地域	United States
City	San Antonio
Period	14/10/26 → 14/10/30

ASJC Scopus subject areas

制御およびシステム工学

他のファイルとリンク

フィンガープリント

「Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

Isokawa, M., Song, Y., Takatsuki, K., Sekiguchi, T., Mizuno, J., Funatsu, T., Shoji, S., & Tsunoda, M. (2014). Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 941-943). Chemical and Biological Microsystems Society.

Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system. / Isokawa, Muneki; Song, Yanting; Takatsuki, Katsuya その他.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 941-943.

研究成果: Conference contribution

Isokawa, M, Song, Y, Takatsuki, K, Sekiguchi, T, Mizuno, J, Funatsu, T, Shoji, S & Tsunoda, M 2014, Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 941-943, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 14/10/26.

Isokawa M, Song Y, Takatsuki K, Sekiguchi T, Mizuno J, Funatsu T その他. Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 941-943

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abstract = "A rapid and quantitative method for the determination of phenylalanine (Phe) and tyrosine (Tyr) in plasma using pressure-driven liquid chromatography (LC) on a chip was developed. After derivatizing Phe and Tyr with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), separation was performed within 140 s on a previously developed integration chip with a pillar array column and a gradient elution system [1,2]. The Phe and Tyr in plasma were quantified and good validation data were obtained. As Phe and Tyr are considered to be important biomarkers of some diseases, especially for newborn screening, the developed method is promising for rapid diagnosis in the clinical field.",

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AU - Isokawa, Muneki

AU - Song, Yanting

AU - Takatsuki, Katsuya

AU - Sekiguchi, Tetsushi

AU - Mizuno, Jun

AU - Funatsu, Takashi

AU - Shoji, Shuichi

AU - Tsunoda, Makoto

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N2 - A rapid and quantitative method for the determination of phenylalanine (Phe) and tyrosine (Tyr) in plasma using pressure-driven liquid chromatography (LC) on a chip was developed. After derivatizing Phe and Tyr with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), separation was performed within 140 s on a previously developed integration chip with a pillar array column and a gradient elution system [1,2]. The Phe and Tyr in plasma were quantified and good validation data were obtained. As Phe and Tyr are considered to be important biomarkers of some diseases, especially for newborn screening, the developed method is promising for rapid diagnosis in the clinical field.

AB - A rapid and quantitative method for the determination of phenylalanine (Phe) and tyrosine (Tyr) in plasma using pressure-driven liquid chromatography (LC) on a chip was developed. After derivatizing Phe and Tyr with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), separation was performed within 140 s on a previously developed integration chip with a pillar array column and a gradient elution system [1,2]. The Phe and Tyr in plasma were quantified and good validation data were obtained. As Phe and Tyr are considered to be important biomarkers of some diseases, especially for newborn screening, the developed method is promising for rapid diagnosis in the clinical field.

KW - Amino acids

KW - Fluorescence

KW - Liquid chromatography

KW - Quantitative determination

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