Fluorescence imaging of Escherichia coli on a rotating optical disk

Takayuki Shima*, Hideyuki Shiramizu, Makoto Fujimaki, Chiaki Kuroda, Yoshimichi Ohki

*この研究の対応する著者

研究成果: Article査読

1 被引用数 (Scopus)

抄録

We constructed a fluorescence image from a rotating optical disk sample by aligning laser-scanned results based on positioning marks formed on the disk substrate. When Escherichia coli cells and 0.75-μm-diameter polystyrene beads were placed on the sample surface and a cell staining solution was used, fluorescence from only E. coli cells was observed in the obtained image. This indicates that E. coli cells can be distinguished from polystyrene beads, an abiotic substance, in one image. A promising utilization of this method is as a sensor for finding targets among contaminants on a large area.

本文言語English
論文番号088003
ジャーナルJapanese journal of applied physics
57
8
DOI
出版ステータスPublished - 2018 8月

ASJC Scopus subject areas

  • 工学一般
  • 物理学および天文学一般

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