抄録
A label-free detection strategy for the detection of platelet-derived growth factor (PDGF-BB) oncoprotein detection using signaling aptamer/protein binding complex is reported. The 3D carbon microarrays detection platform was fabricated by pyrolyzing patterned photoresist and surface functionalized using directamination technique. The detection strategy is based on the release of TOTO intercalating dye from the target binding aptamer's stem structure when it captures PDGF. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration with a very good detection limit of 5 pmol. This detection strategy is promising for the potential detection of different cancer biomarkers and proteins.
| 本文言語 | English |
|---|---|
| ホスト出版物のタイトル | Nano/Bio Sensors |
| 出版社 | Electrochemical Society Inc. |
| ページ | 7-14 |
| ページ数 | 8 |
| 版 | 15 |
| ISBN（電子版） | 9781623320218 |
| ISBN（印刷版） | 9781623320218 |
| DOI | |
| 出版ステータス | Published - 2012 |
| イベント | Symposium on Nano/Bio Sensors - 221st ECS Meeting - Seattle, WA, United States 継続期間: 2012 5月 6 → 2012 5月 10 |
出版物シリーズ
| 名前 | ECS Transactions |
|---|---|
| 番号 | 15 |
| 巻 | 45 |
| ISSN（印刷版） | 1938-5862 |
| ISSN（電子版） | 1938-6737 |
Conference
| Conference | Symposium on Nano/Bio Sensors - 221st ECS Meeting |
|---|---|
| 国/地域 | United States |
| City | Seattle, WA |
| Period | 12/5/6 → 12/5/10 |
ASJC Scopus subject areas
- 工学一般
UN SDG
この成果は、次の持続可能な開発目標に貢献しています
