Generation and characterization of mice lacking gastrin-releasing peptide receptor

Etsuko Wada*, Kei Watase, Kazuyuki Yamada, Hiroo Ogura, Mariko Yamano, Yuji Inomata, Junichi Eguchi, Kazutoshi Yamamoto, Mary E. Sunday, Hiroshi Maeno, Katsuhiko Mikoshiba, Hiroko Ohki-Hamazaki, Keiji Wada

*この研究の対応する著者

    研究成果: Article査読

    68 被引用数 (Scopus)

    抄録

    Gastrin-releasing peptide (GRP) is a mammalian bombesin-like peptide which is widely distributed in the central nervous system as well as in the gastrointestinal tract. GRP binds to its high affinity receptor (GRPR) to elicit a wide spectrum of biological effects on behavior, digestion, and metabolism. To define the in vivo function of GRPR, we generated GRPR null mutant mice by gene targeting. The intracerebroventricular administration of GRP caused hypothermia in wild-type mice, but not in mutant mice. The GRPR deficient mice showed significantly increased locomotor activity during the dark period, and social responses scored by sniffing, mounting, and approaching behaviors against an intruder. Aggressive scores such as fighting and biting were not altered in the mutant mice. These phenotypes were observed in mice generated from two independent ES cell clones and back-crossed to a C57BL/6J background. The GRPR deficient mice should be useful for studying the bombesin system in vivo.

    本文言語English
    ページ(範囲)28-33
    ページ数6
    ジャーナルBiochemical and Biophysical Research Communications
    239
    1
    DOI
    出版ステータスPublished - 1997 10月 9

    ASJC Scopus subject areas

    • 生化学
    • 生物理学
    • 分子生物学

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