TY - JOUR
T1 - High efficiency electrochemical immuno sensors using 3D comb electrodes
AU - Honda, Nobuo
AU - Inaba, Masashi
AU - Katagiri, Takashi
AU - Shoji, Shuichi
AU - Sato, Hirotaka
AU - Homma, Takayuki
AU - Osaka, Tetsuya
AU - Saito, Mikiko
AU - Mizuno, Jun
AU - Wada, Yasuo
N1 - Funding Information:
This work is partly supported by Japan Ministry of Education, Culture, Sports Science & Technology Grant-in-Aid for COE Research and 21st COE of Waseda University, Scientific Research Priority Area (B) No. 13124209 and Japan Society for the Promotion Science Grant-in-Aid for Creative Scientific Research No. 13GS0024. The authors thank the Nanotechnology Support Project of Waseda University for their technical advices.
PY - 2005/5/15
Y1 - 2005/5/15
N2 - To realize highly sensitive electrochemical immunoassays, a micro-fabricated three-dimensional (3D) electrode was fabricated and applied to enzyme immuno assay based on production of a redox species. The dimensions of the electrodes are 10 μm in width and 30 μm in height, with 20 μm spacing in between, and the 30 pairs of anode and cathode electrodes made up a single sensor. This structure lead to enhancement of the electrochemical reaction, nearly 100% of trap ratio of redox species. It can be applied to highly sensitive enzyme immuno sensing based on p-aminophenylphosphate (PAPP). Applicability of this technique to the immuno assay for one of the clinical diagnostic marker proteins (alpha-fetoprotein; AFP) from 6 to 500 ng/mL was demonstrated.
AB - To realize highly sensitive electrochemical immunoassays, a micro-fabricated three-dimensional (3D) electrode was fabricated and applied to enzyme immuno assay based on production of a redox species. The dimensions of the electrodes are 10 μm in width and 30 μm in height, with 20 μm spacing in between, and the 30 pairs of anode and cathode electrodes made up a single sensor. This structure lead to enhancement of the electrochemical reaction, nearly 100% of trap ratio of redox species. It can be applied to highly sensitive enzyme immuno sensing based on p-aminophenylphosphate (PAPP). Applicability of this technique to the immuno assay for one of the clinical diagnostic marker proteins (alpha-fetoprotein; AFP) from 6 to 500 ng/mL was demonstrated.
KW - 3D comb electrode
KW - Amperometric immuno sensors
KW - Electrochemical enzyme immunoassay
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U2 - 10.1016/j.bios.2004.11.007
DO - 10.1016/j.bios.2004.11.007
M3 - Article
C2 - 15797330
AN - SCOPUS:20144388904
SN - 0956-5663
VL - 20
SP - 2306
EP - 2309
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 11
ER -