@article{6e675335a1124239a87b99fda265a4a6,
title = "High sensitivity in vivo imaging of cancer metastasis using a near-infrared luciferin analogue seMpai",
abstract = "Bioluminescence imaging (BLI) is useful to monitor cell movement and gene expression in live animals. However, D-luciferin has a short wavelength (560 nm) which is absorbed by tissues and the use of near-infrared (NIR) luciferin analogues enable high sensitivity in vivo BLI. The AkaLumine-AkaLuc BLI system (Aka-BLI) can detect resolution at the single-cell level; however, it has a clear hepatic background signal. Here, to enable the highly sensitive detection of bioluminescence from the surrounding liver tissues, we focused on seMpai (C15H16N3O2S) which has been synthesized as a luciferin analogue and has high luminescent abilities as same as AkaLumine. We demonstrated that seMpai BLI could detect micro-signals near the liver without any background signal. The solution of seMpai was neutral; therefore, seMpai imaging did not cause any adverse effect in mice. seMpai enabled a highly sensitive in vivo BLI as compared to previous techniques. Our findings suggest that the development of a novel mutated luciferase against seMpai may enable a highly sensitive BLI at the single-cell level without any background signal. Novel seMpai BLI system can be used for in vivo imaging in the fields of life sciences and medicine.",
keywords = "In vivo imaging, Luciferin analogue, Metastasis, Near-infrared bioluminescence",
author = "Jun Nakayama and Ryohei Saito and Yusuke Hayashi and Nobuo Kitada and Shota Tamaki and Yuxuan Han and Kentaro Semba and Maki, {Shojiro A.}",
note = "Funding Information: This study was supported by JSPS KAKENHI (Grant No. 18K16269: Grant-in-Aid for Early-Career Scientist to J.N., Grant No. 20J01794: Grant in Aid for JSPS fellows to J.N., Grant No. JP20K22724: Grant-in-Aid for Research Activity Start-up to R.S., Grant No. JP15H05948: Scientific Research on Innovation Areas “Resonance Bio” to S.A.M.), the Japan Science and Technology Agency (Adaptable and Seamless Technology Transfer Program through Target-driven R&D (A-Step) (No. AS2614119N) to S.A.M.), and the funding received from Kurogane Kasei Co. Ltd. to S.A.M. Funding Information: Funding: This study was supported by JSPS KAKENHI (Grant No. 18K16269: Grant‐in‐Aid for Early‐Career Scientist to J.N., Grant No. 20J01794: Grant in Aid for JSPS fellows to J.N., Grant No. JP20K22724: Grant‐in‐Aid for Research Activity Start‐up to R.S., Grant No. JP15H05948: Scientific Research on Innovation Areas “Resonance Bio” to S.A.M.), the Japan Science and Technology Agency (Adaptable and Seamless Technology Transfer Program through Target‐driven R&D (A‐Step) (No. AS2614119N) to S.A.M.), and the funding received from Kurogane Kasei Co. Ltd. to S.A.M. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2020",
month = nov,
day = "1",
doi = "10.3390/ijms21217896",
language = "English",
volume = "21",
pages = "1--8",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "21",
}