Hyperprocessing of tRNA by the catalytic RNA of RNase P: Cleavage of a natural tRNA within the mature tRNA sequence and evidence for an altered conformation of the substrate tRNA

Yo Kikuchi*, Noriko Sasaki

*この研究の対応する著者

研究成果: Article査読

32 被引用数 (Scopus)

抄録

In the transposon copia-related retrovirus-like particles of Drosophila, a 39-nucleotide-long fragment from the 5′-region of Drosophila initiator methionine tRNA (tRNAi Met) is used as the primer for copia minusstrand reverse transcription. This primer tRNAi Met fragment is thought to be produced by cleavage within the mature tRNAi Met sequence. We call this cleavage hyperprocessing. We have previously reported that catalytic RNA of RNase P from Escherichia coli (MlRNA) cleaves the synthetic tRNAi Met precursor in vitro at several sites within the mature tRNA sequence. Based on this result, we proposed a model for formation of the primer tRNA fragment involving RNase P. Here we show that natural tRNAi Met prepared from DrosophUa adult flies can be cleaved by MIRNA. Using mutant tRNAi Met substrates, we also show that these cleavages are dependent on the occurrence of an altered conformation of the tRNA substrate. This is evidence that a tRNA can exist in aqueous solution at least in part in an altered conformation.

本文言語English
ページ(範囲)11972-11976
ページ数5
ジャーナルJournal of Biological Chemistry
267
17
出版ステータスPublished - 1992 6月 15
外部発表はい

ASJC Scopus subject areas

  • 生化学

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