TY - JOUR
T1 - Imaging of Mitotic Spindle Dynamics in Caenorhabditis elegans Embryos
AU - Toya, Mika
AU - Iida, Yumi
AU - Sugimoto, Asako
N1 - Funding Information:
We thank Geraldine Seydoux, Anjon Audhya, and Karen Oegema for plasmids, Kentaro Nakano for sharing the fixation protocol. We are grateful for Masamitsu Sato and members of the Sugimoto laboratory for discussion, M.T. is a RIKEN Spetial Postdoctoral Researcher and supported by JSPS KAKENHI 21570209. A.S. is supported by MEXT KAKENHI 17017038 and JSPS KAKENHI 19671003.
PY - 2010
Y1 - 2010
N2 - Development of the nematode Caenorhabditis elegans is highly reproducible, and the cell division patterns are virtually invariant. Transparency of the eggshell and cells enables the observation of intracellular events with a high temporal and spatial resolution. These unique features, along with the sophisticated genetic techniques, make this organism one of the most attractive model systems for dissecting regulatory mechanisms of dynamic cellular behaviors, such as mitosis, at an organismal level. In this chapter, we describe immunofluorescence and live imaging methods for analyzing mitotic spindle regulation. In particular, we present the use of double- or triple-labeled fluorescent strains for high-resolution two-dimensional and three-dimensional live imaging to analyze dynamic behaviors of mitotic spindles.
AB - Development of the nematode Caenorhabditis elegans is highly reproducible, and the cell division patterns are virtually invariant. Transparency of the eggshell and cells enables the observation of intracellular events with a high temporal and spatial resolution. These unique features, along with the sophisticated genetic techniques, make this organism one of the most attractive model systems for dissecting regulatory mechanisms of dynamic cellular behaviors, such as mitosis, at an organismal level. In this chapter, we describe immunofluorescence and live imaging methods for analyzing mitotic spindle regulation. In particular, we present the use of double- or triple-labeled fluorescent strains for high-resolution two-dimensional and three-dimensional live imaging to analyze dynamic behaviors of mitotic spindles.
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U2 - 10.1016/S0091-679X(10)97019-2
DO - 10.1016/S0091-679X(10)97019-2
M3 - Article
C2 - 20719280
AN - SCOPUS:77955620597
SN - 0091-679X
VL - 97
SP - 359
EP - 372
JO - Methods in Cell Biology
JF - Methods in Cell Biology
IS - C
ER -