@article{095b23704948400681dc6e83d5f8d29c,
title = "Immobilization of target-bound aptamer on field effect transistor biosensor to improve sensitivity for detection of uncharged cortisol",
abstract = "Field effect transistor (FET) biosensors are capable of detecting various biomolecules, although challenges remain in the detection of uncharged molecules. In this study, the detection of uncharged cortisol was demonstrated by interfacial design using a technique to immobilize target-bound aptamers. The target-bound aptamers, which formed a higher-order structure than target-unbound aptamers, expanded the distance between adjacent aptamers and reduced the steric hindrance to the conformational change. The density-controlled aptamers efficiently induced their conformational changes with the cortisol binding, which resulted in the improvement of the sensitivity of FET biosensors.",
keywords = "Aptamer, Conformational change, Cortisol, Field effect transistor",
author = "Hiroki Hayashi and Ryo Toyama and Ryota Takibuchi and Sho Hideshima and Shigeki Kuroiwa and Naoto Kaneko and Katsunori Horii and Keishi Ohashi and Toshiyuki Momma and Tetsuya Osaka",
note = "Funding Information: This research was supported by JST-COI Program Grant Number, JPMJCE1303, JST-OPERA Program Grant Number, JPMJOP1612, and JST-A-STEP Program no. AS2525029M, all from Japan Science and Technology Agency (JST), Japan. The authors would like to thank Dr. Iwao Waga of NEC Solution Innovators, Ltd. for proposing aptamer-based stress marker sensing and supporting our activities as a leader of the JST-COI and JST-ASTEP programs. Publisher Copyright: {\textcopyright} The Author(s) 2020.",
year = "2021",
month = mar,
day = "5",
doi = "10.5796/electrochemistry.20-00144",
language = "English",
volume = "89",
pages = "134--137",
journal = "Electrochemistry",
issn = "1344-3542",
publisher = "Electrochemical Society of Japan",
number = "2",
}