抄録
We have developed a novel Immunoassay method using bacterial magnetic particles for the determination of immunoglobulin G (IgG). Fluorescein isothiocyanate (FITC) conjugated anti IgG-bacterial magnetic particles were prepared. The fluorescence quenching caused by agglutination of FITC-anti IgG antibody-bacterial magnetic particle conjugates was measured by using a fluorescence spectrophotometer. The aggregates based on specific immunoreaction were separated by a gelatin solution. The aggregation of bacterial magnetic particle conjugates was enhanced by application of a magnetic field. The relative fluorescence intensity correlated linearly with a concentration of IgG in the range 0.5-100 ng/mL.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 268-272 |
| ページ数 | 5 |
| ジャーナル | Analytical Chemistry |
| 巻 | 63 |
| 号 | 3 |
| DOI | |
| 出版ステータス | Published - 1991 2月 1 |
| 外部発表 | はい |
ASJC Scopus subject areas
- 分析化学