TY - JOUR
T1 - Improved passive cell distributing method for micro cellular diagnostic well array
AU - Kawai, Kentaro
AU - Kanai, Masaki
AU - Nakanishi, Hiroaki
AU - Shoji, Shuichi
PY - 2006/6/20
Y1 - 2006/6/20
N2 - We developed a novel biological cell injection method for a large number of diagnostic wells fabricated in parallel. This method facilitates simultaneous cell injection into a microwell array with a simple channel configuration, which can distribute cells evenly to each microwell only by introducing cell suspensions from the inlet. In order to enhance the uniformity of the numbers of injected cells in each microwell, we developed the new concept of flow interchange, which can suppress deviation of the cell flow profile in the cell loading channels. The uniformity of the injected fluorescent beads in each microwell was evaluated using prototype devices of eight microwell arrays. It was confirmed that the flow interchange structure is effective to realize uniform injection. The coefficient of variation of bead numbers in eight microwells was 20.7%.
AB - We developed a novel biological cell injection method for a large number of diagnostic wells fabricated in parallel. This method facilitates simultaneous cell injection into a microwell array with a simple channel configuration, which can distribute cells evenly to each microwell only by introducing cell suspensions from the inlet. In order to enhance the uniformity of the numbers of injected cells in each microwell, we developed the new concept of flow interchange, which can suppress deviation of the cell flow profile in the cell loading channels. The uniformity of the injected fluorescent beads in each microwell was evaluated using prototype devices of eight microwell arrays. It was confirmed that the flow interchange structure is effective to realize uniform injection. The coefficient of variation of bead numbers in eight microwells was 20.7%.
KW - Cell sorting
KW - High throughput screening
KW - Micro well array
KW - Passive distribution
KW - μTAS
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U2 - 10.1143/JJAP.45.5607
DO - 10.1143/JJAP.45.5607
M3 - Review article
AN - SCOPUS:33745655840
SN - 0021-4922
VL - 45
SP - 5607
EP - 5613
JO - Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
JF - Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
IS - 6 B
ER -