Improved passive cell distributing method for micro cellular diagnostic well array

Kentaro Kawai*, Masaki Kanai, Hiroaki Nakanishi, Shuichi Shoji


研究成果: Review article査読

2 被引用数 (Scopus)


We developed a novel biological cell injection method for a large number of diagnostic wells fabricated in parallel. This method facilitates simultaneous cell injection into a microwell array with a simple channel configuration, which can distribute cells evenly to each microwell only by introducing cell suspensions from the inlet. In order to enhance the uniformity of the numbers of injected cells in each microwell, we developed the new concept of flow interchange, which can suppress deviation of the cell flow profile in the cell loading channels. The uniformity of the injected fluorescent beads in each microwell was evaluated using prototype devices of eight microwell arrays. It was confirmed that the flow interchange structure is effective to realize uniform injection. The coefficient of variation of bead numbers in eight microwells was 20.7%.

ジャーナルJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
6 B
出版ステータスPublished - 2006 6月 20

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(全般)


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