Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system

Tomoyasu Atoji; Motonari Aihara; Shinji Takeoka; Eishun Tsuchida

Improvement of oxygen carrying ability of hemoglobin vesicles by H ₂O ₂ elimination system

Tomoyasu Atoji^*, Motonari Aihara, Shinji Takeoka, Eishun Tsuchida

^*この研究の対応する著者

先進理工学部

研究成果: Paper › 査読

抄録

Oxyhemoglobin (oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide (H ₂O ₂) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. Here, we constructed a suppression system of metHb formation within Hb vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb in the Hb vesicles by coencapsulating metHb and L-Tyr on the experiment of stepwise addition of H ₂O ₂ and autoxidation condition. Furthermore, this system was also effective in vivo. We concluded that H ₂O ₂ was effectively eliminated by the metHb/L-Tyr system in the Hb vesicles, and oxygen carrying ability was successfully prolonged.

本文言語	English
ページ	4927-4928
ページ数	2
出版ステータス	Published - 2005 12月 1
イベント	54th SPSJ Symposium on Macromolecules - Yamagata, Japan 継続期間: 2005 9月 20 → 2005 9月 22

Other

Other	54th SPSJ Symposium on Macromolecules
国/地域	Japan
City	Yamagata
Period	05/9/20 → 05/9/22

ASJC Scopus subject areas

工学（全般）

他のファイルとリンク

引用スタイル

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title = "Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system",

abstract = "Oxyhemoglobin (oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide (H 2O 2) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. Here, we constructed a suppression system of metHb formation within Hb vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb in the Hb vesicles by coencapsulating metHb and L-Tyr on the experiment of stepwise addition of H 2O 2 and autoxidation condition. Furthermore, this system was also effective in vivo. We concluded that H 2O 2 was effectively eliminated by the metHb/L-Tyr system in the Hb vesicles, and oxygen carrying ability was successfully prolonged.",

keywords = "Hb vesicles, Hydrogen peroxide/, L-tyrosine, MetHb, Peroxidase activity",

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T1 - Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system

AU - Atoji, Tomoyasu

AU - Aihara, Motonari

AU - Takeoka, Shinji

AU - Tsuchida, Eishun

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Oxyhemoglobin (oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide (H 2O 2) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. Here, we constructed a suppression system of metHb formation within Hb vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb in the Hb vesicles by coencapsulating metHb and L-Tyr on the experiment of stepwise addition of H 2O 2 and autoxidation condition. Furthermore, this system was also effective in vivo. We concluded that H 2O 2 was effectively eliminated by the metHb/L-Tyr system in the Hb vesicles, and oxygen carrying ability was successfully prolonged.

AB - Oxyhemoglobin (oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide (H 2O 2) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. Here, we constructed a suppression system of metHb formation within Hb vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb in the Hb vesicles by coencapsulating metHb and L-Tyr on the experiment of stepwise addition of H 2O 2 and autoxidation condition. Furthermore, this system was also effective in vivo. We concluded that H 2O 2 was effectively eliminated by the metHb/L-Tyr system in the Hb vesicles, and oxygen carrying ability was successfully prolonged.

KW - Hb vesicles

KW - Hydrogen peroxide/

KW - L-tyrosine

KW - MetHb

KW - Peroxidase activity

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T2 - 54th SPSJ Symposium on Macromolecules

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