TY - JOUR
T1 - Induction of Cell Death in Mesothelioma Cells by Magnetite Nanoparticles
AU - Matsuda, Shofu
AU - Hitsuji, Airi
AU - Nakanishi, Takuya
AU - Zhang, Hong
AU - Tanaka, Akane
AU - Matsuda, Hiroshi
AU - Osaka, Tetsuya
N1 - Funding Information:
This work was financially supported by a Grant-in-Aid for Specially Promoted Research (Grant Number 20002006) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, and by a Waseda University Grant for Special Research Projects (2013B-279 and 2014B- 249). Also, this work was partly supported by the Center of Innovation Program from Japan Science and Technology Agency (JST). We are grateful to Mr. Shunpei Asuka (APRO Life Science Institute, Inc.) for help with microarray analysis. S.M. acknowledges the Leading Graduate Program in Science and Engineering, Waseda University, from MEXT, Japan.
Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/12/14
Y1 - 2015/12/14
N2 - Nanoparticle uptake and cell death following addition of magnetite nanoparticles (MNPs) with a diameter of ∼10 nm were evaluated in three histological types of human mesothelioma cells, NCI-H28 (epithelioid), NCI-H2052 (sarcomatoid), and MSTO-211H (biphasic) cells, and human breast cancer MCF-7 cells. Dose-dependent cell death was observed in MSTO-211H cells but not in MCF-7 cells, although cellular uptake of MNPs was observed in both cell types. Mesothelioma NCI-H28 and NCI-H2052 cells showed behavior more similar to that of breast cancer MCF-7 cells than that of mesothelioma MSTO-211H cells. DNA fragmentation and microarray analyses suggested that MNPs induced transforming growth factor β2 related apoptosis in MSTO-211H cells. On the other hand, the viability of human mesothelioma cells containing MNPs with a diameter of ∼40 nm was investigated after exposure to an alternating magnetic field. Temperature increase under the alternating magnetic field and high rates of cell death were observed in all three histological types of human mesothelioma.
AB - Nanoparticle uptake and cell death following addition of magnetite nanoparticles (MNPs) with a diameter of ∼10 nm were evaluated in three histological types of human mesothelioma cells, NCI-H28 (epithelioid), NCI-H2052 (sarcomatoid), and MSTO-211H (biphasic) cells, and human breast cancer MCF-7 cells. Dose-dependent cell death was observed in MSTO-211H cells but not in MCF-7 cells, although cellular uptake of MNPs was observed in both cell types. Mesothelioma NCI-H28 and NCI-H2052 cells showed behavior more similar to that of breast cancer MCF-7 cells than that of mesothelioma MSTO-211H cells. DNA fragmentation and microarray analyses suggested that MNPs induced transforming growth factor β2 related apoptosis in MSTO-211H cells. On the other hand, the viability of human mesothelioma cells containing MNPs with a diameter of ∼40 nm was investigated after exposure to an alternating magnetic field. Temperature increase under the alternating magnetic field and high rates of cell death were observed in all three histological types of human mesothelioma.
KW - cytotoxicity
KW - magnetite nanoparticles
KW - mesothelioma
KW - uptake
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U2 - 10.1021/acsbiomaterials.5b00009
DO - 10.1021/acsbiomaterials.5b00009
M3 - Article
AN - SCOPUS:84969132758
SN - 2373-9878
VL - 1
SP - 632
EP - 638
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
IS - 8
ER -
