TY - JOUR
T1 - Inhibitory effect of Lysichiton camtschatcense extracts on Fe 2+/ascorbate-induced lipid peroxidation in rat kidney and brain homogenates
AU - Takatsu, Hirokatsu
AU - Ogata, Masahiro
AU - Fukui, Koji
AU - Takeda, Tadahiro
AU - Endo, Toyoshige
AU - Urano, Shiro
PY - 2009/7
Y1 - 2009/7
N2 - Lysichiton camtschatcense is a well-known plant in Japan where it has been used as a traditional medicine by the "Ainu" people for the treatment of acute nephritis. It is presumed that L. camtschatcense has an inhibitory effect against nephritis caused by reactive oxygen species (ROS) owing to its antioxidant activities. Consequently, the antioxidant effect of L. camtschatcense extracts was assessed against Fe2+/ascorbic acid (AsA)-induced lipid peroxidation in rat kidney and brain homogenates. The antioxidant effect of the chloroform extract (CE) was more potent than that of the methanol extract (ME) for both homogenates. The antioxidant effect of both extracts was similar to those of α-tocopherol, a lipid-soluble antioxidant, and glutathione (GSH), a water-soluble antioxidant, which were used as reference compounds. Although CE showed a low radical-scavenging effect for superoxide anion radicals (O 2 •- ) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, assessed by using an electron spin resonance (ESR) method, hydroxyl radicals (•OH) were markedly scavenged by more than 80%. On the other hand, ME showed more significant scavenging effect for DPPH radicals and O 2 •- than CE. These results suggest that the inhibitory effects of the L. camtschatcense extract on lipid peroxidation in rat kidney and brain are based on its high radical-scavenging effect against •OH, O 2 •- , and lipid-derived radicals generated from the cell membrane.
AB - Lysichiton camtschatcense is a well-known plant in Japan where it has been used as a traditional medicine by the "Ainu" people for the treatment of acute nephritis. It is presumed that L. camtschatcense has an inhibitory effect against nephritis caused by reactive oxygen species (ROS) owing to its antioxidant activities. Consequently, the antioxidant effect of L. camtschatcense extracts was assessed against Fe2+/ascorbic acid (AsA)-induced lipid peroxidation in rat kidney and brain homogenates. The antioxidant effect of the chloroform extract (CE) was more potent than that of the methanol extract (ME) for both homogenates. The antioxidant effect of both extracts was similar to those of α-tocopherol, a lipid-soluble antioxidant, and glutathione (GSH), a water-soluble antioxidant, which were used as reference compounds. Although CE showed a low radical-scavenging effect for superoxide anion radicals (O 2 •- ) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, assessed by using an electron spin resonance (ESR) method, hydroxyl radicals (•OH) were markedly scavenged by more than 80%. On the other hand, ME showed more significant scavenging effect for DPPH radicals and O 2 •- than CE. These results suggest that the inhibitory effects of the L. camtschatcense extract on lipid peroxidation in rat kidney and brain are based on its high radical-scavenging effect against •OH, O 2 •- , and lipid-derived radicals generated from the cell membrane.
KW - Antioxidant activity
KW - Electron spin resonance
KW - Lipid peroxidation
KW - Lysichiton camtschatcense
KW - Radical scavenging
KW - TBA method
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U2 - 10.1007/s11418-009-0333-9
DO - 10.1007/s11418-009-0333-9
M3 - Article
C2 - 19434471
AN - SCOPUS:67650741755
SN - 1340-3443
VL - 63
SP - 364
EP - 367
JO - Journal of Natural Medicines
JF - Journal of Natural Medicines
IS - 3
ER -