In the present study, we identified the key protein that modulates the initiation of functional glutamatergic synapses in developing cortical neurons. First, we found a day in vitro that marked a critical increase in the number of functional synapses by the application of cyclic AMP, as demonstrated with Ca2+ imaging. We then examined the changes in the expression levels of proteins, which were expected to play a role in glutamatergic synapses, by the application of cyclic AMP. Our findings suggest that the expression of the α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors modulates the initiation of functional synapses in developing neurons, and that immature neurons already contain N-methyl D-aspartate (NMDA) receptors and presynaptic proteins such as synaptophysin.
ASJC Scopus subject areas