Investigation of organelle-specific intracellular water structures with Raman microspectroscopy

Shraeddha Tiwari, Masahiro Ando, Hiro O. Hamaguchi*

*この研究の対応する著者

研究成果: Article査読

8 被引用数 (Scopus)

抄録

The study of intracellular water in living cells is a challenge of fundamental importance. While the critical roles of water in maintaining and propagating life have been widely recognized and accepted, our understanding of water/biomolecule interactions is surprisingly limited. Using Raman microspectroscopic imaging of a living yeast cell followed by a multivariate analysis in form of the nonnegative matrix factorization method, we successfully resolve organelle-specific water structures. The intensities and the band profiles of the segregated water OH stretch spectra yield important and otherwise unobtainable information on the extensive effect of the water/biomolecule interactions in a given organelle on the hydrogen-bonding network of water molecules.

本文言語English
ページ(範囲)167-169
ページ数3
ジャーナルJournal of Raman Spectroscopy
44
2
DOI
出版ステータスPublished - 2013 2月
外部発表はい

ASJC Scopus subject areas

  • 材料科学一般
  • 分光学

フィンガープリント

「Investigation of organelle-specific intracellular water structures with Raman microspectroscopy」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル