TY - JOUR
T1 - Label-free detection of allergens in food via surfactant-induced signal amplification using a field effect transistor-based biosensor
AU - Hideshima, Sho
AU - Saito, Mai
AU - Fujita, Keisuke
AU - Harada, Yoshitaka
AU - Tsuna, Mika
AU - Sekiguchi, Satoshi
AU - Kuroiwa, Shigeki
AU - Nakanishi, Takuya
AU - Osaka, Tetsuya
N1 - Publisher Copyright:
© 2017 The Authors
PY - 2018
Y1 - 2018
N2 - In recent years, food safety concerning food allergy has received increasing attention globally. A simple and sensitive detection method should be developed to measure trace amounts of allergens in foods. Here, we propose a label-free field effect transistor (FET)-based biosensing system for the detection of a buckwheat allergenic protein, BWp16, by surfactant-induced signal amplification. BWp16 could be detected by coupling with an anionic surfactant, sodium dodecyl sulfate (SDS), as this coupling enhanced the net charge of the protein, enough to be detected by FET biosensors. A significant response was observed when the allergen was coupled with SDS, while the responses were decreased or unchanged when it was coupled with a cationic or non-ionic surfactant, suggesting that the SDS coupling maintains the antibody recognition ability of the target allergen, and it would be useful to enhance the sensor responses. The fluorescence spectroscopic measurement revealed that the SDS molecules were successfully coupled with target allergenic protein BWp16, resulting in an increase in its net charge. Furthermore, we demonstrated that the FET biosensor enables specific detection of the allergen in food at the desired concentration levels for food safety analysis, suggesting that it could be used as an alternative food allergen analyzer, both industrially and domestically.
AB - In recent years, food safety concerning food allergy has received increasing attention globally. A simple and sensitive detection method should be developed to measure trace amounts of allergens in foods. Here, we propose a label-free field effect transistor (FET)-based biosensing system for the detection of a buckwheat allergenic protein, BWp16, by surfactant-induced signal amplification. BWp16 could be detected by coupling with an anionic surfactant, sodium dodecyl sulfate (SDS), as this coupling enhanced the net charge of the protein, enough to be detected by FET biosensors. A significant response was observed when the allergen was coupled with SDS, while the responses were decreased or unchanged when it was coupled with a cationic or non-ionic surfactant, suggesting that the SDS coupling maintains the antibody recognition ability of the target allergen, and it would be useful to enhance the sensor responses. The fluorescence spectroscopic measurement revealed that the SDS molecules were successfully coupled with target allergenic protein BWp16, resulting in an increase in its net charge. Furthermore, we demonstrated that the FET biosensor enables specific detection of the allergen in food at the desired concentration levels for food safety analysis, suggesting that it could be used as an alternative food allergen analyzer, both industrially and domestically.
KW - Field effect transistor-based biosensor
KW - Food allergen detection
KW - Food inspection
KW - Intrinsic charge
KW - Ionic surfactant
KW - Signal amplification
UR - http://www.scopus.com/inward/record.url?scp=85026738760&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85026738760&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2017.07.187
DO - 10.1016/j.snb.2017.07.187
M3 - Article
AN - SCOPUS:85026738760
SN - 0925-4005
VL - 254
SP - 1011
EP - 1016
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -