Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells

Masao Noguchi; Takahiro Arakawa; Keiko Sumitomo; Yoshinori Yamaguchi; Shuichi Shoji

doi:10.1109/SENSOR.2009.5285403

Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells

Masao Noguchi^*, Takahiro Arakawa, Keiko Sumitomo, Yoshinori Yamaguchi, Shuichi Shoji

^*この研究の対応する著者

基幹理工学部

研究成果: Conference contribution

抄録

Parallel and sequential manipulation of trapping, labeling and content extraction of single cell was realized using a 2-D simple flow channels. The proposed system consists of main channels, cell trapping pockets, drain channels and single cell content collection channels. We fabricated prototypes having 16 cell trapping pockets and 512 cell trapping pockets. Sequential all single cell handling for COS-1 cell was confirmed in 16 pockets type [1]. The sequential trapping and labeling were achieved in 512 pockets type. Capture rate for COS-1 cell was 83.2% and that for BAT cell was 70.3%. These devices are useful for high throughput single cell analysis.

本文言語	English
ホスト出版物のタイトル	TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
ページ	628-631
ページ数	4
DOI	https://doi.org/10.1109/SENSOR.2009.5285403
出版ステータス	Published - 2009 12月 11
イベント	TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO, United States 継続期間: 2009 6月 21 → 2009 6月 25

出版物シリーズ

名前	TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems

Conference

Conference	TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
国/地域	United States
City	Denver, CO
Period	09/6/21 → 09/6/25

ASJC Scopus subject areas

ハードウェアとアーキテクチャ
電子工学および電気工学

文献へのアクセス

10.1109/SENSOR.2009.5285403

他のファイルとリンク

引用スタイル

Noguchi, M., Arakawa, T., Sumitomo, K., Yamaguchi, Y., & Shoji, S. (2009). Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 628-631). Article 5285403 (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2009.5285403

Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. / Noguchi, Masao; Arakawa, Takahiro; Sumitomo, Keiko その他.
TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. p. 628-631 5285403 (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems).

研究成果: Conference contribution

Noguchi, M, Arakawa, T, Sumitomo, K, Yamaguchi, Y & Shoji, S 2009, Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. in TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems., 5285403, TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems, pp. 628-631, TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems, Denver, CO, United States, 09/6/21. https://doi.org/10.1109/SENSOR.2009.5285403

Noguchi M, Arakawa T, Sumitomo K, Yamaguchi Y, Shoji S. Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. p. 628-631. 5285403. (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems). doi: 10.1109/SENSOR.2009.5285403

Noguchi, Masao ; Arakawa, Takahiro ; Sumitomo, Keiko その他. / Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. pp. 628-631 (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems).

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Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells

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Conference

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