Localization of prolactin receptor in the newt brain

Itaru Hasunuma, Fumiyo Toyoda, Kazutoshi Yamamoto, Masayuki Yamashita, Sakae Kikuyama*


    研究成果: Article査読

    16 被引用数 (Scopus)


    In the male newt Cynops pyrrhogaster, prolactin (PRL) acts directly on the central nervous system and induces courtship behavior. As a step to elucidate the localization of neurons on which PRL acts, we developed a polyclonal antibody against an oligopeptide having a sequence completely identical with a part of the sequence of PRL receptors (PRLRs) of two species of newts, C. pyrrhogaster and C. ensicauda, and performed an immunohistochemical study with this antibody. PRLR-immunoreactive cells were observed in the medial amygdala, anterior preoptic area, magnocellular preoptic nucleus, suprachiasmatic nucleus, nucleus of the periventricular organ, ventral hypothalamic nucleus, and choroid plexus. We also performed in situ hybridization with a 35S-labeled newt PRLR antisense RNA probe and detected signals in the preoptic area and choroid plexus. Colocalization of both PRLR-like immunoreactivity and arginine vasotocin-like or mesotocin-like immunoreactivity was demonstrated in the magnocellular preoptic nucleus. This is the first report of PRLR localization in the amphibian brain.

    ジャーナルCell and Tissue Research
    出版ステータスPublished - 2005 6月

    ASJC Scopus subject areas

    • 解剖学
    • 臨床生化学
    • 細胞生物学


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