TY - JOUR
T1 - MazF endoribonucleolytic toxin conserved in nitrospira specifically cleaves the AACU, AACG, and AAUU motifs
AU - Aoi, Rie
AU - Miyamoto, Tatsuki
AU - Yokota, Akiko
AU - Ota, Yuri
AU - Fujitani, Hirotsugu
AU - Tsuneda, Satoshi
AU - Noda, Naohiro
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/5
Y1 - 2020/5
N2 - MazF is an endoribonucleolytic toxin that cleaves intracellular RNAs in sequence-specific manners. It is liberated in bacterial cells in response to environmental changes and is suggested to contribute to bacterial survival by inducing translational regulation. Thus, determining the cleavage specificity provides insights into the physiological functions of MazF orthologues. Nitrospira, detected in a wide range of environments, is thought to have evolved the ability to cope with their surroundings. To investigate the molecular mechanism of its environmental adaption, a MazF module from Nitrospira strain ND1, which was isolated from the activated sludge of a wastewater treatment plant, is examined in this study. By combining a massive parallel sequencing method and fluorometric assay, we detected that this functional RNA-cleaving toxin specifically recognizes the AACU, AACG, and AAUU motifs. Additionally, statistical analysis suggested that this enzyme regulates various specific functions in order to resist environmental stresses.
AB - MazF is an endoribonucleolytic toxin that cleaves intracellular RNAs in sequence-specific manners. It is liberated in bacterial cells in response to environmental changes and is suggested to contribute to bacterial survival by inducing translational regulation. Thus, determining the cleavage specificity provides insights into the physiological functions of MazF orthologues. Nitrospira, detected in a wide range of environments, is thought to have evolved the ability to cope with their surroundings. To investigate the molecular mechanism of its environmental adaption, a MazF module from Nitrospira strain ND1, which was isolated from the activated sludge of a wastewater treatment plant, is examined in this study. By combining a massive parallel sequencing method and fluorometric assay, we detected that this functional RNA-cleaving toxin specifically recognizes the AACU, AACG, and AAUU motifs. Additionally, statistical analysis suggested that this enzyme regulates various specific functions in order to resist environmental stresses.
KW - MazF
KW - Nitrospira
KW - RNase
KW - Sequence-specificity
KW - Toxin-antitoxin systems
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U2 - 10.3390/toxins12050287
DO - 10.3390/toxins12050287
M3 - Article
C2 - 32365819
AN - SCOPUS:85084276743
SN - 2072-6651
VL - 12
JO - Toxins
JF - Toxins
IS - 5
M1 - 287
ER -