TY - GEN
T1 - Measurement of photosynthesis activity using single synecocystis SP. PCC 6803 on microchambers having gas barrier wall and fluorescence oxygen sensor
AU - Maruyama, Hisataka
AU - Matsuda, Yu
AU - Niimi, Tomohide
AU - Unozumi, Nobuyuki
AU - Nanatani, Kei
AU - Arai, F.
PY - 2012/12/1
Y1 - 2012/12/1
N2 - We developed a novel single cell measurement of photosynthesis activity using fluorescent oxygen sensor in a microchamber with gas barrier wall. We used Synechocystis sp. PCC 6803, which is a series of cyanobacteria. The chamber wall contains oxygen sensor and gas barrier wall to measure the dissolved oxygen concentration in the chamber. The oxygen concentration is changed by aerobic respiration and photosynthesis of Synechocystis. The variation of the oxygen concentration was used for evaluation of aerobic respiration and photosynthesis of single Synechocystis. To achieve precise oxygen measurement, we used our proposed compensation method for photo-degradation of fluorescence. We demonstrated photosynthesis evaluation of single Synechocystis and calculated the photosynthetic performance of sugar production of single Synechocystis.
AB - We developed a novel single cell measurement of photosynthesis activity using fluorescent oxygen sensor in a microchamber with gas barrier wall. We used Synechocystis sp. PCC 6803, which is a series of cyanobacteria. The chamber wall contains oxygen sensor and gas barrier wall to measure the dissolved oxygen concentration in the chamber. The oxygen concentration is changed by aerobic respiration and photosynthesis of Synechocystis. The variation of the oxygen concentration was used for evaluation of aerobic respiration and photosynthesis of single Synechocystis. To achieve precise oxygen measurement, we used our proposed compensation method for photo-degradation of fluorescence. We demonstrated photosynthesis evaluation of single Synechocystis and calculated the photosynthetic performance of sugar production of single Synechocystis.
UR - http://www.scopus.com/inward/record.url?scp=84876513596&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876513596&partnerID=8YFLogxK
U2 - 10.1109/MHS.2012.6492497
DO - 10.1109/MHS.2012.6492497
M3 - Conference contribution
AN - SCOPUS:84876513596
SN - 9781467348126
T3 - 2012 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2012
SP - 476
EP - 478
BT - 2012 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2012
T2 - 23rd Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2012
Y2 - 4 November 2012 through 7 November 2012
ER -