Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling

Takayuki Akimoto; Takashi Ushida; Shigeru Miyaki; Hiroshi Akaogi; Kohei Tsuchiya; Zhen Yan; R. Sanders Williams; Tetsuya Tateishi

doi:10.1016/j.bbrc.2005.01.136

Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling

Takayuki Akimoto^*, Takashi Ushida, Shigeru Miyaki, Hiroshi Akaogi, Kohei Tsuchiya, Zhen Yan, R. Sanders Williams, Tetsuya Tateishi

^*この研究の対応する著者

研究成果: Article › 査読

59 被引用数 (Scopus)

抄録

Myoblasts are able to differentiate into other mesenchymal lineages including adipocytes and osteoblasts. However, it is not known how this differentiation is normally regulated in intact animals and humans. Here, we subjected cultured C2C12 myoblasts to cyclic mechanical stretch (20% elongation) during differentiation into adipocytes. Mechanical stretch inhibited the myoblast-to-adipocyte differentiation significantly, concurrent with an enhanced expression of Wnt10b mRNA. Inhibition of the Wnt signaling by incubation of the myoblasts with a soluble Wnt ligand, sFRP-2, abolished the inhibitory function of mechanical stretch on adipogenesis. These findings provide evidence that mechanical stretch inhibits myoblast-to-adipocyte differentiation possibly through Wnt signaling.

本文言語	English
ページ（範囲）	381-385
ページ数	5
ジャーナル	Biochemical and Biophysical Research Communications
巻	329
号	1
DOI	https://doi.org/10.1016/j.bbrc.2005.01.136
出版ステータス	Published - 2005 4月 1
外部発表	はい

ASJC Scopus subject areas

生物理学
生化学
分子生物学
細胞生物学

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10.1016/j.bbrc.2005.01.136

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title = "Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling",

abstract = "Myoblasts are able to differentiate into other mesenchymal lineages including adipocytes and osteoblasts. However, it is not known how this differentiation is normally regulated in intact animals and humans. Here, we subjected cultured C2C12 myoblasts to cyclic mechanical stretch (20% elongation) during differentiation into adipocytes. Mechanical stretch inhibited the myoblast-to-adipocyte differentiation significantly, concurrent with an enhanced expression of Wnt10b mRNA. Inhibition of the Wnt signaling by incubation of the myoblasts with a soluble Wnt ligand, sFRP-2, abolished the inhibitory function of mechanical stretch on adipogenesis. These findings provide evidence that mechanical stretch inhibits myoblast-to-adipocyte differentiation possibly through Wnt signaling.",

keywords = "Adipocytes, C2C12, Mechanical stress, Mechanical stretch, Myoblasts, Trans-differentiation, Wnt10",

author = "Takayuki Akimoto and Takashi Ushida and Shigeru Miyaki and Hiroshi Akaogi and Kohei Tsuchiya and Zhen Yan and Williams, {R. Sanders} and Tetsuya Tateishi",

note = "Funding Information: We are grateful to Dr. Takashi Sato and Dr. Ping Li for critical comments on the manuscript. This work was supported by a Grant-in-Aid from National Institute of Advanced Industrial Sciences and Technology (AIST), and National Institute of Material Sciences (NIMS), Japan. Takayuki Akimoto was the recipient of a Grant-in Aid for Overseas Research Scholars from the Ministry of Education, Science and Culture of Japan.",

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AU - Akimoto, Takayuki

AU - Ushida, Takashi

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AU - Akaogi, Hiroshi

AU - Tsuchiya, Kohei

AU - Yan, Zhen

AU - Williams, R. Sanders

AU - Tateishi, Tetsuya

N1 - Funding Information: We are grateful to Dr. Takashi Sato and Dr. Ping Li for critical comments on the manuscript. This work was supported by a Grant-in-Aid from National Institute of Advanced Industrial Sciences and Technology (AIST), and National Institute of Material Sciences (NIMS), Japan. Takayuki Akimoto was the recipient of a Grant-in Aid for Overseas Research Scholars from the Ministry of Education, Science and Culture of Japan.

PY - 2005/4/1

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AB - Myoblasts are able to differentiate into other mesenchymal lineages including adipocytes and osteoblasts. However, it is not known how this differentiation is normally regulated in intact animals and humans. Here, we subjected cultured C2C12 myoblasts to cyclic mechanical stretch (20% elongation) during differentiation into adipocytes. Mechanical stretch inhibited the myoblast-to-adipocyte differentiation significantly, concurrent with an enhanced expression of Wnt10b mRNA. Inhibition of the Wnt signaling by incubation of the myoblasts with a soluble Wnt ligand, sFRP-2, abolished the inhibitory function of mechanical stretch on adipogenesis. These findings provide evidence that mechanical stretch inhibits myoblast-to-adipocyte differentiation possibly through Wnt signaling.

KW - Adipocytes

KW - C2C12

KW - Mechanical stress

KW - Mechanical stretch

KW - Myoblasts

KW - Trans-differentiation

KW - Wnt10

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Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling

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