Micro chamber for cellar analysis integrated with negligible dead volume sample injector

Masaki Kanai; Hirohsia Abe; Tatsuya Munaka; Y. Yoichi Fujiyama; Daisuke Uchida; Asako Yamayoshi; Hiroaki Nakanishi; Akira Murakami; Shuichi Shoji

doi:10.1016/j.sna.2003.12.006

Micro chamber for cellar analysis integrated with negligible dead volume sample injector

Masaki Kanai^*, Hirohsia Abe, Tatsuya Munaka, Y. Yoichi Fujiyama, Daisuke Uchida, Asako Yamayoshi, Hiroaki Nakanishi, Akira Murakami, Shuichi Shoji

^*この研究の対応する著者

基幹理工学部

研究成果: Conference article › 査読

20 被引用数 (Scopus)

抄録

A micro chamber integrated with an extremely low dead volume sample injector for real-time monitoring of living cell was developed. By means of the combination of isotropic etching and deep trench etching using CCP-RIE and ICP-RIE, the sample injector with negligible dead volume of less than 0.05nl is realized. Using fabricated devices, the reproducible sample injection of the order of 1nl was obtained. Biological cell culture of rat mast cell was also performed in the fabricated micro chamber.

本文言語	English
ページ（範囲）	129-134
ページ数	6
ジャーナル	Sensors and Actuators, A: Physical
巻	114
号	2-3
DOI	https://doi.org/10.1016/j.sna.2003.12.006
出版ステータス	Published - 2004 9月 1
イベント	Selected Papers from Transducers 03 - Boston, MA, United States 継続期間: 2003 6月 8 → 2003 6月 12

ASJC Scopus subject areas

電子材料、光学材料、および磁性材料
器械工学
凝縮系物理学
表面、皮膜および薄膜
金属および合金
電子工学および電気工学

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10.1016/j.sna.2003.12.006

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title = "Micro chamber for cellar analysis integrated with negligible dead volume sample injector",

abstract = "A micro chamber integrated with an extremely low dead volume sample injector for real-time monitoring of living cell was developed. By means of the combination of isotropic etching and deep trench etching using CCP-RIE and ICP-RIE, the sample injector with negligible dead volume of less than 0.05nl is realized. Using fabricated devices, the reproducible sample injection of the order of 1nl was obtained. Biological cell culture of rat mast cell was also performed in the fabricated micro chamber.",

keywords = "Cell analysis, Cell culture, Microvalve, Sample injector",

author = "Masaki Kanai and Hirohsia Abe and Tatsuya Munaka and Fujiyama, {Y. Yoichi} and Daisuke Uchida and Asako Yamayoshi and Hiroaki Nakanishi and Akira Murakami and Shuichi Shoji",

note = "Funding Information: This work is supported by Research and Development Program for New Bio-Industry Initiatives, of Bio-oriented Technology Research Advancement Institution. This work is partly supported by Japan Ministry of Education, Culture, Sports Science & Technology Grant-in-Aid for COE Research and 21st COE of Waseda University, Scientific Research Priority Area (B) No. 13124209 and Japan Society for the Promotion Science Grant-in-Aid for Creative Scientific Research No. 13GS0024. The authors thank for Nanotechnology Support Project of Waseda University for their technical advices. ; Selected Papers from Transducers 03 ; Conference date: 08-06-2003 Through 12-06-2003",

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doi = "10.1016/j.sna.2003.12.006",

language = "English",

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journal = "Sensors and Actuators, A: Physical",

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TY - JOUR

T1 - Micro chamber for cellar analysis integrated with negligible dead volume sample injector

AU - Kanai, Masaki

AU - Abe, Hirohsia

AU - Munaka, Tatsuya

AU - Fujiyama, Y. Yoichi

AU - Uchida, Daisuke

AU - Yamayoshi, Asako

AU - Nakanishi, Hiroaki

AU - Murakami, Akira

AU - Shoji, Shuichi

N1 - Funding Information: This work is supported by Research and Development Program for New Bio-Industry Initiatives, of Bio-oriented Technology Research Advancement Institution. This work is partly supported by Japan Ministry of Education, Culture, Sports Science & Technology Grant-in-Aid for COE Research and 21st COE of Waseda University, Scientific Research Priority Area (B) No. 13124209 and Japan Society for the Promotion Science Grant-in-Aid for Creative Scientific Research No. 13GS0024. The authors thank for Nanotechnology Support Project of Waseda University for their technical advices.

PY - 2004/9/1

Y1 - 2004/9/1

N2 - A micro chamber integrated with an extremely low dead volume sample injector for real-time monitoring of living cell was developed. By means of the combination of isotropic etching and deep trench etching using CCP-RIE and ICP-RIE, the sample injector with negligible dead volume of less than 0.05nl is realized. Using fabricated devices, the reproducible sample injection of the order of 1nl was obtained. Biological cell culture of rat mast cell was also performed in the fabricated micro chamber.

AB - A micro chamber integrated with an extremely low dead volume sample injector for real-time monitoring of living cell was developed. By means of the combination of isotropic etching and deep trench etching using CCP-RIE and ICP-RIE, the sample injector with negligible dead volume of less than 0.05nl is realized. Using fabricated devices, the reproducible sample injection of the order of 1nl was obtained. Biological cell culture of rat mast cell was also performed in the fabricated micro chamber.

KW - Cell analysis

KW - Cell culture

KW - Microvalve

KW - Sample injector

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UR - http://www.scopus.com/inward/citedby.url?scp=4344585455&partnerID=8YFLogxK

U2 - 10.1016/j.sna.2003.12.006

DO - 10.1016/j.sna.2003.12.006

M3 - Conference article

AN - SCOPUS:4344585455

SN - 0924-4247

VL - 114

SP - 129

EP - 134

JO - Sensors and Actuators, A: Physical

JF - Sensors and Actuators, A: Physical

IS - 2-3

T2 - Selected Papers from Transducers 03

Y2 - 8 June 2003 through 12 June 2003

ER -

Micro chamber for cellar analysis integrated with negligible dead volume sample injector

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ASJC Scopus subject areas

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