Modified ELISA for ultrasensitive diagnosis

Naoko Tsurusawa; Jyunhao Chang; Mayuri Namba; Daiki Makioka; Sou Yamura; Kanako Iha; Yuta Kyosei; Satoshi Watabe; Teruki Yoshimura; Etsuro Ito

doi:10.3390/jcm10215197

Modified ELISA for ultrasensitive diagnosis

Naoko Tsurusawa, Jyunhao Chang, Mayuri Namba, Daiki Makioka, Sou Yamura, Kanako Iha, Yuta Kyosei, Satoshi Watabe, Teruki Yoshimura, Etsuro Ito^*

^*この研究の対応する著者

教育学部

研究成果: Review article › 査読

16 被引用数 (Scopus)

抄録

An enzyme-linked immunosorbent assay (ELISA) can be used for quantitative measurement of proteins, and improving the detection sensitivity to the ultrasensitive level would facilitate the diagnosis of various diseases. In the present review article, we first define the term ‘ultrasensitive’. We follow this with a survey and discussion of the current literature regarding modified ELISA methods with ultrasensitive detection and their application for diagnosis. Finally, we introduce our own newly devised system for ultrasensitive ELISA combined with thionicotinamide adenine dinucleotide cycling and its application for the diagnosis of infectious diseases and lifestylerelated diseases. The aim of the present article is to expand the application of ultrasensitive ELISAs in the medical and biological fields.

本文言語	English
論文番号	5197
ジャーナル	Journal of Clinical Medicine
巻	10
号	21
DOI	https://doi.org/10.3390/jcm10215197
出版ステータス	Published - 2021 11月 1

ASJC Scopus subject areas

医学（全般）

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

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10.3390/jcm10215197

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title = "Modified ELISA for ultrasensitive diagnosis",

abstract = "An enzyme-linked immunosorbent assay (ELISA) can be used for quantitative measurement of proteins, and improving the detection sensitivity to the ultrasensitive level would facilitate the diagnosis of various diseases. In the present review article, we first define the term {\textquoteleft}ultrasensitive{\textquoteright}. We follow this with a survey and discussion of the current literature regarding modified ELISA methods with ultrasensitive detection and their application for diagnosis. Finally, we introduce our own newly devised system for ultrasensitive ELISA combined with thionicotinamide adenine dinucleotide cycling and its application for the diagnosis of infectious diseases and lifestylerelated diseases. The aim of the present article is to expand the application of ultrasensitive ELISAs in the medical and biological fields.",

keywords = "Diagnosis, Infectious disease, Lifestyle-related disease, Thio-NAD cycling, Ultrasensitive ELISA",

author = "Naoko Tsurusawa and Jyunhao Chang and Mayuri Namba and Daiki Makioka and Sou Yamura and Kanako Iha and Yuta Kyosei and Satoshi Watabe and Teruki Yoshimura and Etsuro Ito",

note = "Funding Information: Funding: This research was funded by Grants-in-Aid for Young Scientists (Early Bird) from the Waseda Research Institute for Science and Engineering, Waseda University for K.I. and Y.K., Grants for A-STEP Program from JST (AS3015096U and JPMJTM20LW) for E.I., and a Grant-in-Aid for Scientific Research (KAKENHI) from JSPS (20H04556) for E.I. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = nov,

day = "1",

doi = "10.3390/jcm10215197",

language = "English",

volume = "10",

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T1 - Modified ELISA for ultrasensitive diagnosis

AU - Tsurusawa, Naoko

AU - Chang, Jyunhao

AU - Namba, Mayuri

AU - Makioka, Daiki

AU - Yamura, Sou

AU - Iha, Kanako

AU - Kyosei, Yuta

AU - Watabe, Satoshi

AU - Yoshimura, Teruki

AU - Ito, Etsuro

N1 - Funding Information: Funding: This research was funded by Grants-in-Aid for Young Scientists (Early Bird) from the Waseda Research Institute for Science and Engineering, Waseda University for K.I. and Y.K., Grants for A-STEP Program from JST (AS3015096U and JPMJTM20LW) for E.I., and a Grant-in-Aid for Scientific Research (KAKENHI) from JSPS (20H04556) for E.I. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/11/1

Y1 - 2021/11/1

N2 - An enzyme-linked immunosorbent assay (ELISA) can be used for quantitative measurement of proteins, and improving the detection sensitivity to the ultrasensitive level would facilitate the diagnosis of various diseases. In the present review article, we first define the term ‘ultrasensitive’. We follow this with a survey and discussion of the current literature regarding modified ELISA methods with ultrasensitive detection and their application for diagnosis. Finally, we introduce our own newly devised system for ultrasensitive ELISA combined with thionicotinamide adenine dinucleotide cycling and its application for the diagnosis of infectious diseases and lifestylerelated diseases. The aim of the present article is to expand the application of ultrasensitive ELISAs in the medical and biological fields.

AB - An enzyme-linked immunosorbent assay (ELISA) can be used for quantitative measurement of proteins, and improving the detection sensitivity to the ultrasensitive level would facilitate the diagnosis of various diseases. In the present review article, we first define the term ‘ultrasensitive’. We follow this with a survey and discussion of the current literature regarding modified ELISA methods with ultrasensitive detection and their application for diagnosis. Finally, we introduce our own newly devised system for ultrasensitive ELISA combined with thionicotinamide adenine dinucleotide cycling and its application for the diagnosis of infectious diseases and lifestylerelated diseases. The aim of the present article is to expand the application of ultrasensitive ELISAs in the medical and biological fields.

KW - Diagnosis

KW - Infectious disease

KW - Lifestyle-related disease

KW - Thio-NAD cycling

KW - Ultrasensitive ELISA

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U2 - 10.3390/jcm10215197

DO - 10.3390/jcm10215197

M3 - Review article

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VL - 10

JO - Journal of Clinical Medicine

JF - Journal of Clinical Medicine

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Modified ELISA for ultrasensitive diagnosis

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ASJC Scopus subject areas

UN SDG

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