Molecular cloning and chromosomal localization of the human thrombopoietin gene

Yoshiaki Sohma; Hiromichi Akahori; Naohiko Seki; Tada aki Hori; Kinya Ogami; Takashi Kato; Yoshihiro Shimada; Kazuo Kawamura; Hiroshi Miyazaki

doi:10.1016/0014-5793(94)01008-0

Molecular cloning and chromosomal localization of the human thrombopoietin gene

Yoshiaki Sohma, Hiromichi Akahori, Naohiko Seki, Tada aki Hori, Kinya Ogami, Takashi Kato, Yoshihiro Shimada, Kazuo Kawamura^*, Hiroshi Miyazaki

^*この研究の対応する著者

研究成果: Article › 査読

52 被引用数 (Scopus)

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The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by S1 nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.

本文言語	English
ページ（範囲）	57-61
ページ数	5
ジャーナル	FEBS Letters
巻	353
号	1
DOI	https://doi.org/10.1016/0014-5793(94)01008-0
出版ステータス	Published - 1994 10月 10
外部発表	はい

ASJC Scopus subject areas

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

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10.1016/0014-5793(94)01008-0

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abstract = "The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by S1 nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.",

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AU - Sohma, Yoshiaki

AU - Akahori, Hiromichi

AU - Seki, Naohiko

AU - Hori, Tada aki

AU - Ogami, Kinya

AU - Kato, Takashi

AU - Shimada, Yoshihiro

AU - Kawamura, Kazuo

AU - Miyazaki, Hiroshi

PY - 1994/10/10

Y1 - 1994/10/10

N2 - The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by S1 nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.

AB - The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by S1 nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.

KW - Chromosome mapping

KW - Genomic DNA

KW - Thrombopoietin

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Molecular cloning and chromosomal localization of the human thrombopoietin gene

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