TY - JOUR
T1 - Monoclonal antibody analysis of phosphatidylserine and protein kinase C localizations in developing rat cerebellum
AU - Miyazawa, Atsuo
AU - Inoue, Hiroko
AU - Yoshioka, Tohru
AU - Horikoshi, Tetsuro
AU - Yanagisawa, Keiji
AU - Umeda, Masato
AU - Inoue, Keizo
PY - 1992/10
Y1 - 1992/10
N2 - Understanding the topographical relationships between phosphatidylserine (PS) and protein kinase C (PKC) within neurons can provide clues about the mechanism of translocation and activation of PKC. For this purpose we applied monoclonal antibodies (Abs) of PS and PKC to sections of developing rat cerebellum. The anti-PKC Ab immunohistochemical pattern showed homogeneous staining of Purkinje cells over various postnatal ages, whereas the anti-PS Ab staining showed a heterogeneous localization over these ages. Purkinje cells did not stain well between postnatal day 14 (PND 14) and PND 21, suggesting that the PS was lost from the membrane during preparation of the sections during this period. These data imply that interactions between PS and PKC vary in Purkinje cells during postnatal development.
AB - Understanding the topographical relationships between phosphatidylserine (PS) and protein kinase C (PKC) within neurons can provide clues about the mechanism of translocation and activation of PKC. For this purpose we applied monoclonal antibodies (Abs) of PS and PKC to sections of developing rat cerebellum. The anti-PKC Ab immunohistochemical pattern showed homogeneous staining of Purkinje cells over various postnatal ages, whereas the anti-PS Ab staining showed a heterogeneous localization over these ages. Purkinje cells did not stain well between postnatal day 14 (PND 14) and PND 21, suggesting that the PS was lost from the membrane during preparation of the sections during this period. These data imply that interactions between PS and PKC vary in Purkinje cells during postnatal development.
KW - Anti-phosphatidylserine antibody
KW - Antiprotein kinase C antibody
KW - Cerebellum
KW - Developing Purkinje cell
KW - Immunohistochemical study
KW - Phosphatidylserine-protein kinase C interaction
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M3 - Article
C2 - 1402903
AN - SCOPUS:0026776016
SN - 0022-3042
VL - 59
SP - 1547
EP - 1554
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 4
ER -