Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring

Yukiko Sato; Takahiro Arakawa; Tomoya Sameshima; Taro Ueno; Takashi Funatsu; Shuichi Shoji

Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring

Yukiko Sato, Takahiro Arakawa, Tomoya Sameshima, Taro Ueno, Takashi Funatsu, Shuichi Shoji

基幹理工学部

研究成果: Paper › 査読

1 被引用数 (Scopus)

抄録

We develop a multi-reagent high speed exchange flow system integrated with Total Internal Reflection Fluorescent Microscopy (TIRFM). The reagent switching time within 100 ms was obtained with high performance in-channel PDMS microvalves, the real time monitoring of a single protein behavior, the chaperonin GroEL - cochaperonin GroES rapid interaction, was performed. The fabricated TIRFM microflow system enables the observation of single biomolecular level very short time reactions of a few seconds.

本文言語	English
ページ	603-605
ページ数	3
出版ステータス	Published - 2006 1月 1
イベント	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan 継続期間: 2006 11月 5 → 2006 11月 9

Conference

Conference	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
国/地域	Japan
City	Tokyo
Period	06/11/5 → 06/11/9

ASJC Scopus subject areas

化学工学（その他）
バイオエンジニアリング

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引用スタイル

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title = "Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring",

abstract = "We develop a multi-reagent high speed exchange flow system integrated with Total Internal Reflection Fluorescent Microscopy (TIRFM). The reagent switching time within 100 ms was obtained with high performance in-channel PDMS microvalves, the real time monitoring of a single protein behavior, the chaperonin GroEL - cochaperonin GroES rapid interaction, was performed. The fabricated TIRFM microflow system enables the observation of single biomolecular level very short time reactions of a few seconds.",

keywords = "Microflow system, PDMS microvalves, Protein analysis, Real time monitoring, Single biomolecular detection",

author = "Yukiko Sato and Takahiro Arakawa and Tomoya Sameshima and Taro Ueno and Takashi Funatsu and Shuichi Shoji",

year = "2006",

month = jan,

day = "1",

language = "English",

pages = "603--605",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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TY - CONF

T1 - Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring

AU - Sato, Yukiko

AU - Arakawa, Takahiro

AU - Sameshima, Tomoya

AU - Ueno, Taro

AU - Funatsu, Takashi

AU - Shoji, Shuichi

PY - 2006/1/1

Y1 - 2006/1/1

N2 - We develop a multi-reagent high speed exchange flow system integrated with Total Internal Reflection Fluorescent Microscopy (TIRFM). The reagent switching time within 100 ms was obtained with high performance in-channel PDMS microvalves, the real time monitoring of a single protein behavior, the chaperonin GroEL - cochaperonin GroES rapid interaction, was performed. The fabricated TIRFM microflow system enables the observation of single biomolecular level very short time reactions of a few seconds.

AB - We develop a multi-reagent high speed exchange flow system integrated with Total Internal Reflection Fluorescent Microscopy (TIRFM). The reagent switching time within 100 ms was obtained with high performance in-channel PDMS microvalves, the real time monitoring of a single protein behavior, the chaperonin GroEL - cochaperonin GroES rapid interaction, was performed. The fabricated TIRFM microflow system enables the observation of single biomolecular level very short time reactions of a few seconds.

KW - Microflow system

KW - PDMS microvalves

KW - Protein analysis

KW - Real time monitoring

KW - Single biomolecular detection

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UR - http://www.scopus.com/inward/citedby.url?scp=84902436762&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902436762

SP - 603

EP - 605

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring

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Conference

ASJC Scopus subject areas

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