Multianalyte detection of cancer biomarkers in human serum using label-free field effect transistor biosensor

Ke Si, Shanshan Cheng*, Sho Hideshima, Shigeki Kuroiwa, Takuya Nakanishi, Tetsuya Osaka

*この研究の対応する著者

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Recent statistics show that the incidence and mortality of cancer are on the rise. Among all types of cancer, lung cancer and liver cancer are the most prevalent. We have many biomarkers for these two cancers, among which the cytokeratin fragment 21-1 (CYFRA 21-1) and α-fetoprotein (AFP) are the most common and widely used. Among the various detection methods, the field effect transistor (FET) biosensor is one of the most attractive approaches, providing a label-free, fast, and low-cost electrical detection of biomarkers with high specificity and sensitivity. In previous work, we applied biomarkers for a single cancer type. With the detection of CYFRA 21-1 and neuron-specific enolase (NSE), lung cancer was differentiated in the early stages. Compared with the early work, the biomarkers for the two cancer types, CYFRA 21-1 for lung cancer and AFP for liver cancer, were prepared in this work. The detection of CYFRA 21-1 and AFP in human serum was achieved at the same time with the limits of detection of 1 and 10 ng mL−1, while the cut-off values were 4 and 10 ng mL−1. The method featured short analytical time, small sample volume, and low cost. With its good selectivity and appropriate sensitivity, clinical application will become popular in the future.

本文言語English
ページ(範囲)991-999
ページ数9
ジャーナルSensors and Materials
30
5
DOI
出版ステータスPublished - 2018
外部発表はい

ASJC Scopus subject areas

  • 器械工学
  • 材料科学一般

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