TY - JOUR
T1 - Nanaomycin K, a new epithelial–mesenchymal transition inhibitor produced by the actinomycete “Streptomyces rosa subsp. notoensis” OS-3966
AU - Matsuo, Hirotaka
AU - Nakanishi, Jun
AU - Noguchi, Yoshihiko
AU - Kitagawa, Koichi
AU - Shigemura, Katsumi
AU - Sunazuka, Toshiaki
AU - Takahashi, Yōko
AU - Ōmura, Satoshi
AU - Nakashima, Takuji
N1 - Funding Information:
The authors declare no conflict of interest. This study was supported by funds from the Institute for Fermentation, Osaka (IFO), Japan.
Publisher Copyright:
© 2019 The Society for Biotechnology, Japan
PY - 2020/3
Y1 - 2020/3
N2 - A new nanaomycin analog, nanaomycin K, was isolated from a cultured broth of actinomycete strain “Streptomyces rosa subsp. notoensis” OS-3966. Nuclear magnetic resonance (NMR) analyses revealed that the planar structure of nanaomycin K had an ergothioneine moiety. To determine the absolute configuration, nanaomycin K was semisynthesized using standards of nanaomycin E and L-ergothioneine. The natural and semisynthetic nanaomycin K were identified as the same compounds based on retention time, mass spectrometry, 1H NMR, and optical rotation data. Nanaomycin K showed cytotoxicity against Madin–Darby canine kidney (MDCK) cells undergoing transforming growth factor (TGF) β1-induced epithelial–mesenchymal transition.
AB - A new nanaomycin analog, nanaomycin K, was isolated from a cultured broth of actinomycete strain “Streptomyces rosa subsp. notoensis” OS-3966. Nuclear magnetic resonance (NMR) analyses revealed that the planar structure of nanaomycin K had an ergothioneine moiety. To determine the absolute configuration, nanaomycin K was semisynthesized using standards of nanaomycin E and L-ergothioneine. The natural and semisynthetic nanaomycin K were identified as the same compounds based on retention time, mass spectrometry, 1H NMR, and optical rotation data. Nanaomycin K showed cytotoxicity against Madin–Darby canine kidney (MDCK) cells undergoing transforming growth factor (TGF) β1-induced epithelial–mesenchymal transition.
KW - Actinomycete
KW - Epithelial–mesenchymal transition
KW - Ergothioneine
KW - Nanaomycin K
KW - Transforming growth factor β1
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U2 - 10.1016/j.jbiosc.2019.09.007
DO - 10.1016/j.jbiosc.2019.09.007
M3 - Article
C2 - 31582334
AN - SCOPUS:85072710121
SN - 1389-1723
VL - 129
SP - 291
EP - 295
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 3
ER -