@article{4a5fa66945524a2b8a52610237029457,
title = "Nanogelation and Thermal Stabilization of Enzyme by Vitamin B6-Bearing Polysaccharide as Biocrosslinker",
abstract = "Nanogels containing a protein (carbonic anhydrase, CA) were prepared by cross-linking CA and pyridoxal (vitamin B6)-bearing pullulan (PLPP) as a biocrosslinker via Schiff base formation. UV titration and high-performance liquid chromatography confirmed that CA was quantitatively complexed with PLPP in the presence of zinc ions. Dynamic light scattering and transmission electron microscopy showed that the nanogel diameter was about 20 nm. CA retained 90% of its native activity after complexation with PLPP. Moreover, the residual enzymatic activity of CA after heating and its long-term storage stability at room temperature were improved by complexation with PLPP. Enzyme nanogelation with PLPP is an efficient method for enzyme stabilization.",
keywords = "biocrosslinker, enzyme, nanogel, polysaccharide, pyridoxal",
author = "Yoshihiro Sasaki and Yuji Tsuchido and Takahiro Yoshimura and Kazunari Akiyoshi",
note = "Funding Information: T.Y. gratefully acknowledges Graduate School of Engineering, Kyoto University for support of graduate studies. The authors also acknowledge the contribution of Assistant Professor Shin-ichi Sawada for support of SEC measurements. This work was funded by a grant for Exploratory Research for Advanced Technology of the Japan Science and Technology Agency (JST-ERATO). This work was also supported by the Grant-in Aid from the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant JP16H06313 (K. A.) and JP16H03842 (Y.S.). Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",
year = "2019",
month = nov,
day = "11",
doi = "10.1021/acsbiomaterials.9b00026",
language = "English",
volume = "5",
pages = "5752--5758",
journal = "ACS Biomaterials Science and Engineering",
issn = "2373-9878",
publisher = "American Chemical Society",
number = "11",
}